Galectin-3 Precipitates as a Pentamer with Synthetic Multivalent Carbohydrates and Forms Heterogeneous Cross-linked Complexes

Nisar Ahmad, Hans J. Gabius, Sabine André, Herbert Kaltner, Subramanian Sabesan, René Roy, Bingcan Liu, Frank P. Macaluso, Curtis F. Brewer

Research output: Contribution to journalArticle

357 Citations (Scopus)

Abstract

Galectin-3 is unique among the galectin family of animal lectins in its biological activities and structure. Most members of the galectin family including galectin-1 possess apoptotic activities, whereas galectin-3 possesses anti-apoptotic activity. Galectin-3 is also the only chimera type galectin and consists of a nonlectin N-terminal domain and a C-terminal carbohydrate-binding domain. Recent sedimentation equilibrium and velocity studies show that murine galectin-3 is a monomer in the absence and presence of LacNAc, a monovalent sugar. However, quantitative precipitation studies in the present report indicate that galectin-3 precipitates as a pentamer with a series of divalent pentasaccharides with terminal LacNAc residues. Furthermore, the kinetics of precipitation are fast, on the order of seconds. This indicates that although the majority of galectin-3 in solution is a monomer, a rapid equilibrium exists between the monomer and a small percentage of pentamer. The latter, in turn, precipitates with the divalent oligosaccharides, resulting in rapid conversion of monomer to pentamer by mass action equilibria. Mixed quantitative precipitation experiments and electron microscopy suggest that galectin-3 forms heterogenous, disorganized cross-linking complexes with the multivalent carbohydrates. This contrasts with galectin-1 and many plant lectins that form homogeneous, organized cross-linked complexes. The results are discussed in terms of the biological properties of galectin-3.

Original languageEnglish (US)
Pages (from-to)10841-10847
Number of pages7
JournalJournal of Biological Chemistry
Volume279
Issue number12
DOIs
StatePublished - Mar 19 2004

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Galectin 3
Precipitates
Carbohydrates
Galectins
Monomers
Galectin 1
Plant Lectins
Bioactivity
Oligosaccharides
Sedimentation
Lectins
Sugars
Electron microscopy
Electron Microscopy
Kinetics

ASJC Scopus subject areas

  • Biochemistry

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Galectin-3 Precipitates as a Pentamer with Synthetic Multivalent Carbohydrates and Forms Heterogeneous Cross-linked Complexes. / Ahmad, Nisar; Gabius, Hans J.; André, Sabine; Kaltner, Herbert; Sabesan, Subramanian; Roy, René; Liu, Bingcan; Macaluso, Frank P.; Brewer, Curtis F.

In: Journal of Biological Chemistry, Vol. 279, No. 12, 19.03.2004, p. 10841-10847.

Research output: Contribution to journalArticle

Ahmad, Nisar ; Gabius, Hans J. ; André, Sabine ; Kaltner, Herbert ; Sabesan, Subramanian ; Roy, René ; Liu, Bingcan ; Macaluso, Frank P. ; Brewer, Curtis F. / Galectin-3 Precipitates as a Pentamer with Synthetic Multivalent Carbohydrates and Forms Heterogeneous Cross-linked Complexes. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 12. pp. 10841-10847.
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