Abstract
Galactocerebrosides (GCs) represent a major class of glycolipids in the nervous system. Here, we show that mice lacking the key enzyme to generate GCs, UDP-galactose:ceramide galactosyltransferase (CGT-/-), exhibit severe postnatal atrophy of all lymphoid organs, owing to a maturational arrest before the pro-B/T cell stage. This lineage-specific defect originates from the bone marrow (BM) stroma since it is not transplantable to irradiated wild-type recipients. Remarkably, CGT-/- long-term B lymphoid BM cultures displayed severe deficits in the number of CD45negVCAM-1pos stromal cells and fibronectin matrix assembly, and produced floating macrophages rather than B lymphocytes. The fibronectin network was also altered in the CGT-deficient BM parenchyma. These results point to an essential role for galactolipids in the formation of fibronectin-enriched lymphoid-specific stromal niches in the BM.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 789-800 |
| Number of pages | 12 |
| Journal | Immunity |
| Volume | 18 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1 2003 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
- Immunology
Cite this
Galactocerebrosides are required postnatally for stromal-dependent bone marrow lymphopoiesis. / Katayama, Yoshio; Frenette, Paul S.
In: Immunity, Vol. 18, No. 6, 01.06.2003, p. 789-800.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Galactocerebrosides are required postnatally for stromal-dependent bone marrow lymphopoiesis
AU - Katayama, Yoshio
AU - Frenette, Paul S.
PY - 2003/6/1
Y1 - 2003/6/1
N2 - Galactocerebrosides (GCs) represent a major class of glycolipids in the nervous system. Here, we show that mice lacking the key enzyme to generate GCs, UDP-galactose:ceramide galactosyltransferase (CGT-/-), exhibit severe postnatal atrophy of all lymphoid organs, owing to a maturational arrest before the pro-B/T cell stage. This lineage-specific defect originates from the bone marrow (BM) stroma since it is not transplantable to irradiated wild-type recipients. Remarkably, CGT-/- long-term B lymphoid BM cultures displayed severe deficits in the number of CD45negVCAM-1pos stromal cells and fibronectin matrix assembly, and produced floating macrophages rather than B lymphocytes. The fibronectin network was also altered in the CGT-deficient BM parenchyma. These results point to an essential role for galactolipids in the formation of fibronectin-enriched lymphoid-specific stromal niches in the BM.
AB - Galactocerebrosides (GCs) represent a major class of glycolipids in the nervous system. Here, we show that mice lacking the key enzyme to generate GCs, UDP-galactose:ceramide galactosyltransferase (CGT-/-), exhibit severe postnatal atrophy of all lymphoid organs, owing to a maturational arrest before the pro-B/T cell stage. This lineage-specific defect originates from the bone marrow (BM) stroma since it is not transplantable to irradiated wild-type recipients. Remarkably, CGT-/- long-term B lymphoid BM cultures displayed severe deficits in the number of CD45negVCAM-1pos stromal cells and fibronectin matrix assembly, and produced floating macrophages rather than B lymphocytes. The fibronectin network was also altered in the CGT-deficient BM parenchyma. These results point to an essential role for galactolipids in the formation of fibronectin-enriched lymphoid-specific stromal niches in the BM.
UR - http://www.scopus.com/inward/record.url?scp=0038771245&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038771245&partnerID=8YFLogxK
U2 - 10.1016/S1074-7613(03)00150-X
DO - 10.1016/S1074-7613(03)00150-X
M3 - Article
C2 - 12818160
AN - SCOPUS:0038771245
VL - 18
SP - 789
EP - 800
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 6
ER -