Gabaergic and glutaminergic neurons in the primate's zona incerta

S. M. Owen, D. K. Donahoe, T. P. Ma

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The zona incerta (ZI) of macaque monkeys contains two morphological cell types, local circuit and projection neurons. Physiological study has demonstrated that these cells pause for saccades. As the ZI has a large projection to the superior colliculus (SC) where saccades are generated we hypothesized that the ZI provides a disinhibitory signal which permits saccades to be generated by the SC. To test this hypothesis, we examined whether the ZI contained GABA-immunoreactive neurons. Seven cynomolgus monkeys (Macaca fascicularis) were perfused with mixed aldehyde fixatives. Fifty μm frozen sections were cut and incubated with primary antisera to a variety of neurotransmitters. Subsequently, labeling was visualized using the ABC technique and the sections were counterstained with thionin. Approximately 200 each of immunoreactive and non-immunoreactive cells from the same sections, representing the full range of somatic dimensions within the ZI, were-drawn under high power and somatic dimensions were calculated with a computerized morphometric program (SigmaScan). GABA-immunoreactive cells ranged from 9.5-16 μm long axis and Nissl-stained non-immunoreactive cells ranged from 9.8-20 μm long axis, Glutamate-immunoreactive neurons ranged from 9.7-21.8 μm and non-immunoreactive neurons ranged from 8.2-18.7 μ. From these data we conclude that some ZI projection neurons are probably GABAergic. However, the largest projection neurons in the ZI are not GABAergic and are likely to be glutaminergic. Thus it is unlikely that the ZI disinhibits the SC only through a GABAergic projection. Rather, the ZI is likely to provide both a direct inhibition through a GABAergic projection onto tectoefferent cells and an indirect inhibition through a glutaminergic projection onto tectal local circuit neurons.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

GABAergic Neurons
Primates
Neurons
neurons
Eye movements
Superior Colliculi
Saccades
Macaca fascicularis
gamma-aminobutyric acid
cells
gamma-Aminobutyric Acid
Thionins
Fixatives
Networks (circuits)
neurotransmitters
Zona Incerta
Macaca
Aldehydes
glutamates
Labeling

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Owen, S. M., Donahoe, D. K., & Ma, T. P. (1997). Gabaergic and glutaminergic neurons in the primate's zona incerta. FASEB Journal, 11(3).

Gabaergic and glutaminergic neurons in the primate's zona incerta. / Owen, S. M.; Donahoe, D. K.; Ma, T. P.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Owen, SM, Donahoe, DK & Ma, TP 1997, 'Gabaergic and glutaminergic neurons in the primate's zona incerta', FASEB Journal, vol. 11, no. 3.
Owen, S. M. ; Donahoe, D. K. ; Ma, T. P. / Gabaergic and glutaminergic neurons in the primate's zona incerta. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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