G-protein βγ dimers: Membrane targeting requires subunit coexpression and intact γ C-A-A-X domain

William F. Simonds, James E. Butrynski, Narasimhan Gautam, Cecilia G. Unson, Allen M. Spiegel

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

Attachment of heterotrimeric G-proteins to the inner face of the plasma membrane is fundamental to their role as signal transducers by allowing interaction with both receptors and effectors. Certain G-protein α subunits are anchored to the membrane by covalent myristoylation. The βγ complex is required for G-protein interaction with receptors and is independently membrane associated through an unknown mechanism. A series of carboxyl-terminal modifications including isoprenylation which may contribute to membrane attachment has been identified recently in G-protein γ subunits. Expression and membrane targeting of β and γ subunits were examined in COS cells. The expression of either subunit was found to require cotransfection with both β and γ cDNAs. Mutation of the carboxyl-terminal cysteine residue of γ shown to undergo isoprenylation and carboxymethyl-esterification preserved βγ expression but blocked isoprenylation and membrane attachment. These results implicate the carboxyl-terminal processing of G-protein γ subunits and β coexpression as necessary and sufficient for membrane targeting of the βγ complex.

Original languageEnglish (US)
Pages (from-to)5363-5366
Number of pages4
JournalJournal of Biological Chemistry
Volume266
Issue number9
StatePublished - 1991
Externally publishedYes

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GTP-Binding Proteins
Dimers
Prenylation
Membranes
Protein Subunits
Heterotrimeric GTP-Binding Proteins
Esterification
COS Cells
Cell membranes
Transducers
Cysteine
Complementary DNA
Cell Membrane
Mutation
Processing

ASJC Scopus subject areas

  • Biochemistry

Cite this

G-protein βγ dimers : Membrane targeting requires subunit coexpression and intact γ C-A-A-X domain. / Simonds, William F.; Butrynski, James E.; Gautam, Narasimhan; Unson, Cecilia G.; Spiegel, Allen M.

In: Journal of Biological Chemistry, Vol. 266, No. 9, 1991, p. 5363-5366.

Research output: Contribution to journalArticle

Simonds, William F. ; Butrynski, James E. ; Gautam, Narasimhan ; Unson, Cecilia G. ; Spiegel, Allen M. / G-protein βγ dimers : Membrane targeting requires subunit coexpression and intact γ C-A-A-X domain. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 9. pp. 5363-5366.
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