Fyn phosphorylates human MAP-2c on tyrosine 67

S. Pilar Zamora-Leon, Anne Bresnick, Jonathan M. Backer, Bridget Shafit-Zagardo

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The Src homology 3 (SH3)domain of Fyn binds to a conserved PXXP motif on microtubule-associated protein-2. Co-transfections into COS7 cells and in vitro kinase assays performed with Fyn and wild-type, or mutant MAP-2c, determined that Fyn phosphorylated MAP-2c on tyrosine 67. The phosphorylation generated a consensus sequence for the binding of the SH2 domain of Grb2 (pYSN). Pull-down assays with SH2-Grb2 from human fetal brain homogenates, and co-immunoprecipitation of Grb2 and MAP-2 confirmed the interaction in vivo, and demonstrated that MAP-2c is tyrosine-phosphorylateti in human fetal brain. Filter overlay assays confirmed that the SH2 domain of Grb2 binds to human MAP-2c following incubation with active Fyn. Enzyme-linked immunosorbent assays confirmed the interaction between the SH2 domain of Grb2 and a tyrosine-phosphorylated MAP-2 peptide spanning the PY67SN motif. Thus, MAP-2c can directly recruit multiple signaling proteins important for central nervous system development.

Original languageEnglish (US)
Pages (from-to)1962-1970
Number of pages9
JournalJournal of Biological Chemistry
Volume280
Issue number3
DOIs
StatePublished - Jan 21 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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