Further studies on the factor in lung conditioned medium stimulating granulocyte and monocyte colony formation in vitro

J. W. Sheridan, D. Metcalf, E. R. Stanley

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The Colony Stimulating Factors (CSF) extracted from 6 hr 'in vivo' postendotoxin mouse lung tissue and 48 hr 'in vitro' lung conditioned medium appear to be glycoproteins, for like human urinary CSF they were inactivated by proteases and altered in charge but not inactivated by sialidase. Neither endotoxin injection nor length of in vitro incubation significantly affected the apparent S20(w) (approximately 2.0S) of the bulk of the CSF in mouse lung conditioned medium. The lower apparent mol wt of CSF in mouse lung conditioned medium contrasted with that of monkey lung conditioned medium (S20(w) approximately 3.3S). CSF's in both monkey and mouse lung conditioned media exhibited a much higher apparent MW on gel filtration than one would expect from their sedimentation behavior, a characteristic generally observed with most unpurified CSF preparations. Mouse lung conditioned medium CSF was antigenically distinguishable from other CSF's. Failure to demonstrate this form of CSF in the tissue serum or urine of normal or endotoxin injected mice suggests that this type of CSF might normally lack in vivo significance.

Original languageEnglish (US)
Pages (from-to)147-158
Number of pages12
JournalJournal of Cellular Physiology
Volume84
Issue number1
StatePublished - 1974
Externally publishedYes

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Colony-Stimulating Factors
Conditioned Culture Medium
Granulocytes
Monocytes
Lung
Endotoxins
Haplorhini
Tissue
In Vitro Techniques
Neuraminidase
Sedimentation
Gel Chromatography
Glycoproteins
Peptide Hydrolases
Gels
Urine
Injections

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Further studies on the factor in lung conditioned medium stimulating granulocyte and monocyte colony formation in vitro. / Sheridan, J. W.; Metcalf, D.; Stanley, E. R.

In: Journal of Cellular Physiology, Vol. 84, No. 1, 1974, p. 147-158.

Research output: Contribution to journalArticle

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