Functional identification and structure determination of two novel prolidases from cog1228 in the amidohydrolase superfamily

Dao Feng Xiang, Yury Patskovsky, Chengfu Xu, Alexander A. Fedorov, Elena V. Fedorov, Abby A. Sisco, J. Michael Sauder, Stephen K. Burley, Steven C. Almo, Frank M. Raushel

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Two uncharacterized enzymes from the amidohydrolase superfamily belonging to cog1228 were cloned, expressed, and purified to homogeneity. The two proteins, Sgx9260c (gi|44242006) and Sgx9260b (gi|44479596), were derived from environmental DNA samples originating from the Sargasso Sea. The catalytic function and substrate profiles for Sgx9260c and Sgx9260b were determined using a comprehensive library of dipeptides and N-acyl derivative of l-amino acids. Sgx9260c catalyzes the hydrolysis of Gly-l-Pro, l-Ala-l-Pro, and N-acyl derivatives of l-Pro. The best substrate identified to date is N-acetyl-l-Pro with a value of kcat/Km of 3 × 105 M -1 s-1. Sgx9260b catalyzes the hydrolysis of l-hydrophobic l-Pro dipeptides and N-acyl derivatives of l-Pro. The best substrate identified to date is N-propionyl-l-Pro with a value of kcat/Km of 1 × 105 M-1 s-1. Three-dimensional structures of both proteins were determined by X-ray diffraction methods (PDB codes 3MKV and 3FEQ). These proteins fold as distorted (β/α) 8-barrels with two divalent cations in the active site. The structure of Sgx9260c was also determined as a complex with the N-methylphosphonate derivative of l-Pro (PDB code 3N2C). In this structure the phosphonate moiety bridges the binuclear metal center, and one oxygen atom interacts with His-140. The α-carboxylate of the inhibitor interacts with Tyr-231. The proline side chain occupies a small substrate binding cavity formed by residues contributed from the loop that follows β-strand 7 within the (β/α)8-barrel. A total of 38 other proteins from cog1228 are predicted to have the same substrate profile based on conservation of the substrate binding residues. The structure of an evolutionarily related protein, Cc2672 from Caulobacter crecentus, was determined as a complex with the N-methylphosphonate derivative of l-arginine (PDB code 3MTW).

Original languageEnglish (US)
Pages (from-to)6791-6803
Number of pages13
JournalBiochemistry
Volume49
Issue number31
DOIs
StatePublished - Aug 10 2010

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proline dipeptidase
Amidohydrolases
Derivatives
Substrates
Dipeptides
Proteins
Hydrolysis
Caulobacter
Organophosphonates
Divalent Cations
Proline
X-Ray Diffraction
Arginine
Catalytic Domain
Metals
Conservation
Oxygen
Amino Acids
DNA
X ray diffraction

ASJC Scopus subject areas

  • Biochemistry

Cite this

Xiang, D. F., Patskovsky, Y., Xu, C., Fedorov, A. A., Fedorov, E. V., Sisco, A. A., ... Raushel, F. M. (2010). Functional identification and structure determination of two novel prolidases from cog1228 in the amidohydrolase superfamily. Biochemistry, 49(31), 6791-6803. https://doi.org/10.1021/bi100897u

Functional identification and structure determination of two novel prolidases from cog1228 in the amidohydrolase superfamily. / Xiang, Dao Feng; Patskovsky, Yury; Xu, Chengfu; Fedorov, Alexander A.; Fedorov, Elena V.; Sisco, Abby A.; Sauder, J. Michael; Burley, Stephen K.; Almo, Steven C.; Raushel, Frank M.

In: Biochemistry, Vol. 49, No. 31, 10.08.2010, p. 6791-6803.

Research output: Contribution to journalArticle

Xiang, DF, Patskovsky, Y, Xu, C, Fedorov, AA, Fedorov, EV, Sisco, AA, Sauder, JM, Burley, SK, Almo, SC & Raushel, FM 2010, 'Functional identification and structure determination of two novel prolidases from cog1228 in the amidohydrolase superfamily', Biochemistry, vol. 49, no. 31, pp. 6791-6803. https://doi.org/10.1021/bi100897u
Xiang, Dao Feng ; Patskovsky, Yury ; Xu, Chengfu ; Fedorov, Alexander A. ; Fedorov, Elena V. ; Sisco, Abby A. ; Sauder, J. Michael ; Burley, Stephen K. ; Almo, Steven C. ; Raushel, Frank M. / Functional identification and structure determination of two novel prolidases from cog1228 in the amidohydrolase superfamily. In: Biochemistry. 2010 ; Vol. 49, No. 31. pp. 6791-6803.
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AU - Sisco, Abby A.

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N2 - Two uncharacterized enzymes from the amidohydrolase superfamily belonging to cog1228 were cloned, expressed, and purified to homogeneity. The two proteins, Sgx9260c (gi|44242006) and Sgx9260b (gi|44479596), were derived from environmental DNA samples originating from the Sargasso Sea. The catalytic function and substrate profiles for Sgx9260c and Sgx9260b were determined using a comprehensive library of dipeptides and N-acyl derivative of l-amino acids. Sgx9260c catalyzes the hydrolysis of Gly-l-Pro, l-Ala-l-Pro, and N-acyl derivatives of l-Pro. The best substrate identified to date is N-acetyl-l-Pro with a value of kcat/Km of 3 × 105 M -1 s-1. Sgx9260b catalyzes the hydrolysis of l-hydrophobic l-Pro dipeptides and N-acyl derivatives of l-Pro. The best substrate identified to date is N-propionyl-l-Pro with a value of kcat/Km of 1 × 105 M-1 s-1. Three-dimensional structures of both proteins were determined by X-ray diffraction methods (PDB codes 3MKV and 3FEQ). These proteins fold as distorted (β/α) 8-barrels with two divalent cations in the active site. The structure of Sgx9260c was also determined as a complex with the N-methylphosphonate derivative of l-Pro (PDB code 3N2C). In this structure the phosphonate moiety bridges the binuclear metal center, and one oxygen atom interacts with His-140. The α-carboxylate of the inhibitor interacts with Tyr-231. The proline side chain occupies a small substrate binding cavity formed by residues contributed from the loop that follows β-strand 7 within the (β/α)8-barrel. A total of 38 other proteins from cog1228 are predicted to have the same substrate profile based on conservation of the substrate binding residues. The structure of an evolutionarily related protein, Cc2672 from Caulobacter crecentus, was determined as a complex with the N-methylphosphonate derivative of l-arginine (PDB code 3MTW).

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