Functional heterogeneity of calcium release by inositol trisphosphate in single Purkinje neurones, cultured cerebellar astrocytes, and peripheral tissues

Kamran Khodakhah, David Ogden

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Purkinje neurones of the cerebellar cortex are rich in receptors for the Ca-mobilizing second messenger inositol trisphosphate (InsP3) in association with intracellular Ca stores. Cytosolic Ca ions are important in regulating neuronal excitability but it has proved difficult to demonstrate InsP3-evoked release of Ca in mammalian central neurones directly. Intracellular release of InsP3 by flash photolysis of caged InsP3, combined with whole-cell patch clamp and microspectrofluorimetry of Ca indicators, allows comparison of InsP3-evoked Ca release in single Purkinje cells in cerebellar slices with the same process in cultured astrocytes and peripheral tissues. In astrocytes, hepatocytes, exocrine cells, and vascular endothelium, minimal Ca release from stores requires photorelease of InsP3 at concentrations of 0.2-0.5 μM, and maximal efflux as judged by the rate of increase of Ca concentration is seen with 5-10 μM InsP3. In contrast in Purkinje cells, InsP3 concentrations of ≥9 μM were required to produce minimal Ca release from stores under the same conditions, and Ca efflux increased with InsP3 concentrations up to 70-80 μM. Furthermore, the rate of increase and size of the Ca concentration in Purkinje cells are 10- to 30-fold greater than in astrocytes and peripheral tissues. The InsP3 sensitivity was not affected by changing exogenous cytosolic Ca buffering, suggesting that endogenous Ca binding cannot account for the difference. The results show a functional difference in InsP3-evoked Ca release between Purkinje cells and peripheral tissues.

Original languageEnglish (US)
Pages (from-to)4976-4980
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number11
StatePublished - Jun 1 1993
Externally publishedYes

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Purkinje Cells
Inositol
Astrocytes
Calcium
Cerebellar Cortex
Photolysis
Vascular Endothelium
Second Messenger Systems
Hepatocytes
Ions
Neurons

Keywords

  • Flash photolysis

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "Purkinje neurones of the cerebellar cortex are rich in receptors for the Ca-mobilizing second messenger inositol trisphosphate (InsP3) in association with intracellular Ca stores. Cytosolic Ca ions are important in regulating neuronal excitability but it has proved difficult to demonstrate InsP3-evoked release of Ca in mammalian central neurones directly. Intracellular release of InsP3 by flash photolysis of caged InsP3, combined with whole-cell patch clamp and microspectrofluorimetry of Ca indicators, allows comparison of InsP3-evoked Ca release in single Purkinje cells in cerebellar slices with the same process in cultured astrocytes and peripheral tissues. In astrocytes, hepatocytes, exocrine cells, and vascular endothelium, minimal Ca release from stores requires photorelease of InsP3 at concentrations of 0.2-0.5 μM, and maximal efflux as judged by the rate of increase of Ca concentration is seen with 5-10 μM InsP3. In contrast in Purkinje cells, InsP3 concentrations of ≥9 μM were required to produce minimal Ca release from stores under the same conditions, and Ca efflux increased with InsP3 concentrations up to 70-80 μM. Furthermore, the rate of increase and size of the Ca concentration in Purkinje cells are 10- to 30-fold greater than in astrocytes and peripheral tissues. The InsP3 sensitivity was not affected by changing exogenous cytosolic Ca buffering, suggesting that endogenous Ca binding cannot account for the difference. The results show a functional difference in InsP3-evoked Ca release between Purkinje cells and peripheral tissues.",
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N2 - Purkinje neurones of the cerebellar cortex are rich in receptors for the Ca-mobilizing second messenger inositol trisphosphate (InsP3) in association with intracellular Ca stores. Cytosolic Ca ions are important in regulating neuronal excitability but it has proved difficult to demonstrate InsP3-evoked release of Ca in mammalian central neurones directly. Intracellular release of InsP3 by flash photolysis of caged InsP3, combined with whole-cell patch clamp and microspectrofluorimetry of Ca indicators, allows comparison of InsP3-evoked Ca release in single Purkinje cells in cerebellar slices with the same process in cultured astrocytes and peripheral tissues. In astrocytes, hepatocytes, exocrine cells, and vascular endothelium, minimal Ca release from stores requires photorelease of InsP3 at concentrations of 0.2-0.5 μM, and maximal efflux as judged by the rate of increase of Ca concentration is seen with 5-10 μM InsP3. In contrast in Purkinje cells, InsP3 concentrations of ≥9 μM were required to produce minimal Ca release from stores under the same conditions, and Ca efflux increased with InsP3 concentrations up to 70-80 μM. Furthermore, the rate of increase and size of the Ca concentration in Purkinje cells are 10- to 30-fold greater than in astrocytes and peripheral tissues. The InsP3 sensitivity was not affected by changing exogenous cytosolic Ca buffering, suggesting that endogenous Ca binding cannot account for the difference. The results show a functional difference in InsP3-evoked Ca release between Purkinje cells and peripheral tissues.

AB - Purkinje neurones of the cerebellar cortex are rich in receptors for the Ca-mobilizing second messenger inositol trisphosphate (InsP3) in association with intracellular Ca stores. Cytosolic Ca ions are important in regulating neuronal excitability but it has proved difficult to demonstrate InsP3-evoked release of Ca in mammalian central neurones directly. Intracellular release of InsP3 by flash photolysis of caged InsP3, combined with whole-cell patch clamp and microspectrofluorimetry of Ca indicators, allows comparison of InsP3-evoked Ca release in single Purkinje cells in cerebellar slices with the same process in cultured astrocytes and peripheral tissues. In astrocytes, hepatocytes, exocrine cells, and vascular endothelium, minimal Ca release from stores requires photorelease of InsP3 at concentrations of 0.2-0.5 μM, and maximal efflux as judged by the rate of increase of Ca concentration is seen with 5-10 μM InsP3. In contrast in Purkinje cells, InsP3 concentrations of ≥9 μM were required to produce minimal Ca release from stores under the same conditions, and Ca efflux increased with InsP3 concentrations up to 70-80 μM. Furthermore, the rate of increase and size of the Ca concentration in Purkinje cells are 10- to 30-fold greater than in astrocytes and peripheral tissues. The InsP3 sensitivity was not affected by changing exogenous cytosolic Ca buffering, suggesting that endogenous Ca binding cannot account for the difference. The results show a functional difference in InsP3-evoked Ca release between Purkinje cells and peripheral tissues.

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