Functional genomics reveals the sole sulphate transporter of the Mycobacterium tuberculosis complex and its relevance to the acquisition of sulphur in vivo

Esen Wooff, Stephen Ll Michell, Stephen V. Gordon, Mark A. Chambers, Stoyan Bardarov, William R. Jacobs, R. Glyn Hewinson, Paul R. Wheeler

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Sulphur is essential for some of the most vital biological activities such as translation initiation and redox maintenance, and genes involved in sulphur metabolism have been implicated in virulence. Mycobacterium tuberculosis has three predicted genes for the prototrophic acquisition of sulphur as sulphate: cysA, part of an ABC transporter, and cysA2 and A3, SseC sulphotransferases. Screening for amino acid auxotrophs of Mycobacterium bovis BCG, obtained by transposon mutagenesis, was used to select methionine auxotrophs requiring a sulphur-containing amino acid for growth. We have characterized one of these auxotrophs as being disrupted in cysA. Both the cysA mutant and a previously identified mutant in an upstream gene, subI, were functionally characterized as being completely unable to take up sulphate. Complementation of the cysA mutant with the wild-type gene from M. tuberculosis restored prototrophy and the ability to take up sulphate with the functional characteristics of an ABC transporter. Hence, it appears that this is the sole locus encoding inorganic sulphur transport in the M. tuberculosis complex.

Original languageEnglish (US)
Pages (from-to)653-663
Number of pages11
JournalMolecular Microbiology
Volume43
Issue number3
DOIs
StatePublished - Jun 17 2002

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Genomics
Mycobacterium tuberculosis
Sulfur
Sulfates
ATP-Binding Cassette Transporters
Mycobacterium bovis
Genes
Sulfur Amino Acids
Sulfotransferases
Mutagenesis
Methionine
Oxidation-Reduction
Virulence
Maintenance
Amino Acids
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Functional genomics reveals the sole sulphate transporter of the Mycobacterium tuberculosis complex and its relevance to the acquisition of sulphur in vivo. / Wooff, Esen; Michell, Stephen Ll; Gordon, Stephen V.; Chambers, Mark A.; Bardarov, Stoyan; Jacobs, William R.; Hewinson, R. Glyn; Wheeler, Paul R.

In: Molecular Microbiology, Vol. 43, No. 3, 17.06.2002, p. 653-663.

Research output: Contribution to journalArticle

Wooff, Esen ; Michell, Stephen Ll ; Gordon, Stephen V. ; Chambers, Mark A. ; Bardarov, Stoyan ; Jacobs, William R. ; Hewinson, R. Glyn ; Wheeler, Paul R. / Functional genomics reveals the sole sulphate transporter of the Mycobacterium tuberculosis complex and its relevance to the acquisition of sulphur in vivo. In: Molecular Microbiology. 2002 ; Vol. 43, No. 3. pp. 653-663.
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