Functional expression of the new gap junction gene connexin47 transcribed in mouse brain and spinal cord neurons

Barbara Teubner, Benjamin Odermatt, Martin Güldenagel, Goran Söhl, Joachim Degen, Feliksas F. Bukauskas, Jack Kronengold, Vytas K. Verselis, Yong Tae Jung, Christine A. Kozak, Karl Schilling, Klaus Willecke

Research output: Contribution to journalArticle

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Abstract

A new mouse gap junction gene that codes for a protein of 46,551 Da has been identified and designated connexin47 (Cx47). It mapped as a single-copy gene to mouse chromosome 11. In human HeLa cells and Xenopus oocytes, expression of mouse Cx47 or a fusion protein of Cx47 and enhanced green fluorescent protein induced intercellular channels that displayed strong sensitivity to transjunctional voltage. Tracer injections in Cx47-transfected HeLa cells revealed intercellular diffusion of neurobiotin, Lucifer yellow, and 4′,6-diamidino-2-phenylindole. Recordings of single channels yielded a unitary conductance of 55 pS main state and 8 pS substate. Cx47 mRNA expression was high in spinal cord and brain but was not found in retina, liver, heart, and lung. A low level of Cx47 expression was detected in ovaries. In situ hybridizations demonstrated high expression in α motor neurons of the spinal cord, pyramidal cells of the cortex and hippocampus, granular and molecular layers of the dentate gyrus, and Purkinje cells of the cerebellum as well as several nuclei of the brainstem. This expression pattern is distinct from, although partially overlapping with, that of the neuronally expressed connexin36 gene. Thus, electrical synapses in adult mammalian brain are likely to consist of different connexin proteins depending on the neuronal subtype.

Original languageEnglish (US)
Pages (from-to)1117-1126
Number of pages10
JournalJournal of Neuroscience
Volume21
Issue number4
Publication statusPublished - Feb 15 2001

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Keywords

  • Cx47
  • Electrical synapses
  • Gap junctions
  • Neuronal connexin
  • Permeability
  • Voltage gating

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Teubner, B., Odermatt, B., Güldenagel, M., Söhl, G., Degen, J., Bukauskas, F. F., ... Willecke, K. (2001). Functional expression of the new gap junction gene connexin47 transcribed in mouse brain and spinal cord neurons. Journal of Neuroscience, 21(4), 1117-1126.