Functional expression of the murine connexin 36 gene coding for a neuron-specific gap junctional protein

B. Teubner, J. Degen, G. Söhl, M. Güldenagel, F. F. Bukauskas, E. B. Trexler, Vytautas Verselis, C. I. De Zeeuw, C. G. Lee, C. A. Kozak, E. Petrasch-Parwez, R. Dermietzel, K. Willecke

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

The mouse connexin 36 (Cx36) gene was mapped on chromosome 2 and an identical transcriptional start site was determined in brain and retina on exon I. Rabbit polyclonal antibodies to the presumptive cytoplasmic loop of the Cx36 protein recognized in immunohistochemical analyses Cx36 expression in the retina, olfactory bulb, hippocampus, inferior olive and cerebellum. In olivary neurons strong punctate labeling at dendritic cell contacts and weaker labeling in the cytoplasm of dendrites were shown by immuno electron microscopy. After expression of mouse Cx36 cDNA in human HeLa cells, neurobiotin transfer was increased 1.8-fold and electrical conductance at least 15-fold compared to untransfected HeLa cells. No Lucifer Yellow transfer was detected in either untransfected or Cx36 transfected HeLa cells. Single Cx36 channels in transfected HeLa cells showed a unitary conductance of 14.3 ± 0.8 pS. The sensitivity of Cx36 channels to transjunctional voltage was low in both HeLa-Cx36 cells and Xenopus oocytes expressing mouse Cx36. No increased transfer of neurobiotin was detected in heterotypic gap junctions formed by Cx36 and 9 other connexins expressed in HeLa cells. Our results suggest that Cx36 channels function as electrical synapses for transmission of electrical and metabolic signals between neurons in the central nervous system.

Original languageEnglish (US)
Pages (from-to)249-262
Number of pages14
JournalJournal of Membrane Biology
Volume176
Issue number3
DOIs
StatePublished - 2000

Fingerprint

Neurons
Genes
HeLa Cells
Proteins
Retina
connexin 36
Electrical Synapses
Connexins
Chromosomes, Human, Pair 2
Immunoelectron Microscopy
Olfactory Bulb
Gap Junctions
Dendrites
Xenopus
Dendritic Cells
Cerebellum
Oocytes
Exons
Hippocampus
Cytoplasm

Keywords

  • Cx36
  • Electrical synapses
  • Gap junctions
  • Neuronal connexin
  • Transcriptional start site

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

Cite this

Teubner, B., Degen, J., Söhl, G., Güldenagel, M., Bukauskas, F. F., Trexler, E. B., ... Willecke, K. (2000). Functional expression of the murine connexin 36 gene coding for a neuron-specific gap junctional protein. Journal of Membrane Biology, 176(3), 249-262. https://doi.org/10.1007/s002320001094

Functional expression of the murine connexin 36 gene coding for a neuron-specific gap junctional protein. / Teubner, B.; Degen, J.; Söhl, G.; Güldenagel, M.; Bukauskas, F. F.; Trexler, E. B.; Verselis, Vytautas; De Zeeuw, C. I.; Lee, C. G.; Kozak, C. A.; Petrasch-Parwez, E.; Dermietzel, R.; Willecke, K.

In: Journal of Membrane Biology, Vol. 176, No. 3, 2000, p. 249-262.

Research output: Contribution to journalArticle

Teubner, B, Degen, J, Söhl, G, Güldenagel, M, Bukauskas, FF, Trexler, EB, Verselis, V, De Zeeuw, CI, Lee, CG, Kozak, CA, Petrasch-Parwez, E, Dermietzel, R & Willecke, K 2000, 'Functional expression of the murine connexin 36 gene coding for a neuron-specific gap junctional protein', Journal of Membrane Biology, vol. 176, no. 3, pp. 249-262. https://doi.org/10.1007/s002320001094
Teubner, B. ; Degen, J. ; Söhl, G. ; Güldenagel, M. ; Bukauskas, F. F. ; Trexler, E. B. ; Verselis, Vytautas ; De Zeeuw, C. I. ; Lee, C. G. ; Kozak, C. A. ; Petrasch-Parwez, E. ; Dermietzel, R. ; Willecke, K. / Functional expression of the murine connexin 36 gene coding for a neuron-specific gap junctional protein. In: Journal of Membrane Biology. 2000 ; Vol. 176, No. 3. pp. 249-262.
@article{744afaee9b85440c8bcdca26b4840b6e,
title = "Functional expression of the murine connexin 36 gene coding for a neuron-specific gap junctional protein",
abstract = "The mouse connexin 36 (Cx36) gene was mapped on chromosome 2 and an identical transcriptional start site was determined in brain and retina on exon I. Rabbit polyclonal antibodies to the presumptive cytoplasmic loop of the Cx36 protein recognized in immunohistochemical analyses Cx36 expression in the retina, olfactory bulb, hippocampus, inferior olive and cerebellum. In olivary neurons strong punctate labeling at dendritic cell contacts and weaker labeling in the cytoplasm of dendrites were shown by immuno electron microscopy. After expression of mouse Cx36 cDNA in human HeLa cells, neurobiotin transfer was increased 1.8-fold and electrical conductance at least 15-fold compared to untransfected HeLa cells. No Lucifer Yellow transfer was detected in either untransfected or Cx36 transfected HeLa cells. Single Cx36 channels in transfected HeLa cells showed a unitary conductance of 14.3 ± 0.8 pS. The sensitivity of Cx36 channels to transjunctional voltage was low in both HeLa-Cx36 cells and Xenopus oocytes expressing mouse Cx36. No increased transfer of neurobiotin was detected in heterotypic gap junctions formed by Cx36 and 9 other connexins expressed in HeLa cells. Our results suggest that Cx36 channels function as electrical synapses for transmission of electrical and metabolic signals between neurons in the central nervous system.",
keywords = "Cx36, Electrical synapses, Gap junctions, Neuronal connexin, Transcriptional start site",
author = "B. Teubner and J. Degen and G. S{\"o}hl and M. G{\"u}ldenagel and Bukauskas, {F. F.} and Trexler, {E. B.} and Vytautas Verselis and {De Zeeuw}, {C. I.} and Lee, {C. G.} and Kozak, {C. A.} and E. Petrasch-Parwez and R. Dermietzel and K. Willecke",
year = "2000",
doi = "10.1007/s002320001094",
language = "English (US)",
volume = "176",
pages = "249--262",
journal = "Journal of Membrane Biology",
issn = "0022-2631",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Functional expression of the murine connexin 36 gene coding for a neuron-specific gap junctional protein

AU - Teubner, B.

AU - Degen, J.

AU - Söhl, G.

AU - Güldenagel, M.

AU - Bukauskas, F. F.

AU - Trexler, E. B.

AU - Verselis, Vytautas

AU - De Zeeuw, C. I.

AU - Lee, C. G.

AU - Kozak, C. A.

AU - Petrasch-Parwez, E.

AU - Dermietzel, R.

AU - Willecke, K.

PY - 2000

Y1 - 2000

N2 - The mouse connexin 36 (Cx36) gene was mapped on chromosome 2 and an identical transcriptional start site was determined in brain and retina on exon I. Rabbit polyclonal antibodies to the presumptive cytoplasmic loop of the Cx36 protein recognized in immunohistochemical analyses Cx36 expression in the retina, olfactory bulb, hippocampus, inferior olive and cerebellum. In olivary neurons strong punctate labeling at dendritic cell contacts and weaker labeling in the cytoplasm of dendrites were shown by immuno electron microscopy. After expression of mouse Cx36 cDNA in human HeLa cells, neurobiotin transfer was increased 1.8-fold and electrical conductance at least 15-fold compared to untransfected HeLa cells. No Lucifer Yellow transfer was detected in either untransfected or Cx36 transfected HeLa cells. Single Cx36 channels in transfected HeLa cells showed a unitary conductance of 14.3 ± 0.8 pS. The sensitivity of Cx36 channels to transjunctional voltage was low in both HeLa-Cx36 cells and Xenopus oocytes expressing mouse Cx36. No increased transfer of neurobiotin was detected in heterotypic gap junctions formed by Cx36 and 9 other connexins expressed in HeLa cells. Our results suggest that Cx36 channels function as electrical synapses for transmission of electrical and metabolic signals between neurons in the central nervous system.

AB - The mouse connexin 36 (Cx36) gene was mapped on chromosome 2 and an identical transcriptional start site was determined in brain and retina on exon I. Rabbit polyclonal antibodies to the presumptive cytoplasmic loop of the Cx36 protein recognized in immunohistochemical analyses Cx36 expression in the retina, olfactory bulb, hippocampus, inferior olive and cerebellum. In olivary neurons strong punctate labeling at dendritic cell contacts and weaker labeling in the cytoplasm of dendrites were shown by immuno electron microscopy. After expression of mouse Cx36 cDNA in human HeLa cells, neurobiotin transfer was increased 1.8-fold and electrical conductance at least 15-fold compared to untransfected HeLa cells. No Lucifer Yellow transfer was detected in either untransfected or Cx36 transfected HeLa cells. Single Cx36 channels in transfected HeLa cells showed a unitary conductance of 14.3 ± 0.8 pS. The sensitivity of Cx36 channels to transjunctional voltage was low in both HeLa-Cx36 cells and Xenopus oocytes expressing mouse Cx36. No increased transfer of neurobiotin was detected in heterotypic gap junctions formed by Cx36 and 9 other connexins expressed in HeLa cells. Our results suggest that Cx36 channels function as electrical synapses for transmission of electrical and metabolic signals between neurons in the central nervous system.

KW - Cx36

KW - Electrical synapses

KW - Gap junctions

KW - Neuronal connexin

KW - Transcriptional start site

UR - http://www.scopus.com/inward/record.url?scp=0033843444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033843444&partnerID=8YFLogxK

U2 - 10.1007/s002320001094

DO - 10.1007/s002320001094

M3 - Article

VL - 176

SP - 249

EP - 262

JO - Journal of Membrane Biology

JF - Journal of Membrane Biology

SN - 0022-2631

IS - 3

ER -