Functional expression of the guinea pig 11b-hydroxylase in cos-1 cells

Hannes E. Buelow, Katharina Möbius, Volker Bähr, Rita Bernhardt

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We expressed a guinea pig 11β-hydroxylase cDNA (1) in COS-1 cells. In order to find the optimal expression system we compared three expression plasmids, each driven by a different promoter. Although promoters exhibited different transcriptional activities this did not result in different enzymatic activities. Upon cotransfection with bovine adrenodoxin a 5-fold increase of enzyme activity was achieved. A comparison with the bovine 11β- hydroxylase clearly demonstrated that the guinea pig enzyme was not able to produce significant amounts of 18-hydroxylated and 18-oxidized products from deoxycorticosterone under the experimental conditions used.

Original languageEnglish (US)
Pages (from-to)479-484
Number of pages6
JournalEndocrine Research
Volume22
Issue number4
StatePublished - 1996
Externally publishedYes

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Mixed Function Oxygenases
Guinea Pigs
Adrenodoxin
Desoxycorticosterone
COS Cells
Enzymes
Plasmids
Complementary DNA

ASJC Scopus subject areas

  • Endocrinology

Cite this

Buelow, H. E., Möbius, K., Bähr, V., & Bernhardt, R. (1996). Functional expression of the guinea pig 11b-hydroxylase in cos-1 cells. Endocrine Research, 22(4), 479-484.

Functional expression of the guinea pig 11b-hydroxylase in cos-1 cells. / Buelow, Hannes E.; Möbius, Katharina; Bähr, Volker; Bernhardt, Rita.

In: Endocrine Research, Vol. 22, No. 4, 1996, p. 479-484.

Research output: Contribution to journalArticle

Buelow, HE, Möbius, K, Bähr, V & Bernhardt, R 1996, 'Functional expression of the guinea pig 11b-hydroxylase in cos-1 cells', Endocrine Research, vol. 22, no. 4, pp. 479-484.
Buelow, Hannes E. ; Möbius, Katharina ; Bähr, Volker ; Bernhardt, Rita. / Functional expression of the guinea pig 11b-hydroxylase in cos-1 cells. In: Endocrine Research. 1996 ; Vol. 22, No. 4. pp. 479-484.
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