Functional expression of murine multidrug resistance in Xenopus laevis oocytes

Gonzalo Castillo, Juan C. Vera, Chia Ping Huang Yang, Susan Band Horwitz, Ora M. Rosen

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The development of multidrug resistance (MDR) is associated with the overproduction of a plasma membrane glycoprotein, P glycoprotein. Here we report the functional expression of a member of the murine mdr family of proteins and show that Xenopus oocytes injected with RNA encoding the mouse mdr1b P glycoprotein develop a MDR-like phenotype. Immunological analysis indicated that oocytes injected with the mdr 1b RNA synthesized a protein with the size and immunological characteristics of the mouse mdr 1b P glycoprotein. These oocytes exhibited a decreased accumulation of [3H]vinblastine and showed an increased capacity to extrude the drug compared to control oocytes not expressing the P glycoprotein. In addition, competition experiments indicated that verapamil, vincristine, daunomycin, and quinidine, but not colchicine, can overcome the rapid drug efflux conferred by the expression of the mouse P glycoprotein. (.

Original languageEnglish (US)
Pages (from-to)4737-4741
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number12
DOIs
StatePublished - 1990

Keywords

  • Multidrug transporter
  • P glycoprotein
  • mRNA expression

ASJC Scopus subject areas

  • General

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