Abstract
The development of multidrug resistance (MDR) is associated with the overproduction of a plasma membrane glycoprotein, P glycoprotein. Here we report the functional expression of a member of the murine mdr family of proteins and show that Xenopus oocytes injected with RNA encoding the mouse mdr1b P glycoprotein develop a MDR-like phenotype. Immunological analysis indicated that oocytes injected with the mdr 1b RNA synthesized a protein with the size and immunological characteristics of the mouse mdr 1b P glycoprotein. These oocytes exhibited a decreased accumulation of [3H]vinblastine and showed an increased capacity to extrude the drug compared to control oocytes not expressing the P glycoprotein. In addition, competition experiments indicated that verapamil, vincristine, daunomycin, and quinidine, but not colchicine, can overcome the rapid drug efflux conferred by the expression of the mouse P glycoprotein. (.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4737-4741 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 87 |
| Issue number | 12 |
| State | Published - 1990 |
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Keywords
- mRNA expression
- Multidrug transporter
- P glycoprotein
ASJC Scopus subject areas
- Genetics
- General
Cite this
Functional expression of murine multidrug resistance in Xenopus laevis oocytes. / Castillo, Gonzalo; Vera, Juan C.; Yang, Chia-Ping H.; Band Horwitz, Susan; Rosen, Ora M.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 12, 1990, p. 4737-4741.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional expression of murine multidrug resistance in Xenopus laevis oocytes
AU - Castillo, Gonzalo
AU - Vera, Juan C.
AU - Yang, Chia-Ping H.
AU - Band Horwitz, Susan
AU - Rosen, Ora M.
PY - 1990
Y1 - 1990
N2 - The development of multidrug resistance (MDR) is associated with the overproduction of a plasma membrane glycoprotein, P glycoprotein. Here we report the functional expression of a member of the murine mdr family of proteins and show that Xenopus oocytes injected with RNA encoding the mouse mdr1b P glycoprotein develop a MDR-like phenotype. Immunological analysis indicated that oocytes injected with the mdr 1b RNA synthesized a protein with the size and immunological characteristics of the mouse mdr 1b P glycoprotein. These oocytes exhibited a decreased accumulation of [3H]vinblastine and showed an increased capacity to extrude the drug compared to control oocytes not expressing the P glycoprotein. In addition, competition experiments indicated that verapamil, vincristine, daunomycin, and quinidine, but not colchicine, can overcome the rapid drug efflux conferred by the expression of the mouse P glycoprotein. (.
AB - The development of multidrug resistance (MDR) is associated with the overproduction of a plasma membrane glycoprotein, P glycoprotein. Here we report the functional expression of a member of the murine mdr family of proteins and show that Xenopus oocytes injected with RNA encoding the mouse mdr1b P glycoprotein develop a MDR-like phenotype. Immunological analysis indicated that oocytes injected with the mdr 1b RNA synthesized a protein with the size and immunological characteristics of the mouse mdr 1b P glycoprotein. These oocytes exhibited a decreased accumulation of [3H]vinblastine and showed an increased capacity to extrude the drug compared to control oocytes not expressing the P glycoprotein. In addition, competition experiments indicated that verapamil, vincristine, daunomycin, and quinidine, but not colchicine, can overcome the rapid drug efflux conferred by the expression of the mouse P glycoprotein. (.
KW - mRNA expression
KW - Multidrug transporter
KW - P glycoprotein
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UR - http://www.scopus.com/inward/citedby.url?scp=0025339169&partnerID=8YFLogxK
M3 - Article
C2 - 1693776
AN - SCOPUS:0025339169
VL - 87
SP - 4737
EP - 4741
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 12
ER -