Abstract
The development of multidrug resistance (MDR) is associated with the overproduction of a plasma membrane glycoprotein, P glycoprotein. Here we report the functional expression of a member of the murine mdr family of proteins and show that Xenopus oocytes injected with RNA encoding the mouse mdr1b P glycoprotein develop a MDR-like phenotype. Immunological analysis indicated that oocytes injected with the mdr 1b RNA synthesized a protein with the size and immunological characteristics of the mouse mdr 1b P glycoprotein. These oocytes exhibited a decreased accumulation of [3H]vinblastine and showed an increased capacity to extrude the drug compared to control oocytes not expressing the P glycoprotein. In addition, competition experiments indicated that verapamil, vincristine, daunomycin, and quinidine, but not colchicine, can overcome the rapid drug efflux conferred by the expression of the mouse P glycoprotein. (.
Original language | English (US) |
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Pages (from-to) | 4737-4741 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 87 |
Issue number | 12 |
DOIs | |
State | Published - 1990 |
Keywords
- Multidrug transporter
- P glycoprotein
- mRNA expression
ASJC Scopus subject areas
- General