Functional diversity of the phosphoglucomutase superfamily: Structural implications

Sergei Levin, Steven C. Almo, Birgit H. Satir

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Three-dimensional structural models of three members of the phosphoglucomutase (PGM) superfamify, parafusin, phosphoglucomutase-related protein and sarcoplasmic reticulum phosphoglucomutase, were constructed by homology modeling based on the known crystal structure of rabbit muscle phosphoglucomutase. Parafusin, phosphoglucomutase-related protein and sarcoplasmic reticulum phosphoglucomutase each have 50% or more identity with rabbit muscle phosphoglucomutase at the amino acid level and all are reported to exhibit no or minor phosphoglucomutase activity. There are four major insertions and two deletions in the parafusin sequence relative to PGM, all of which are located in surface-exposed loops connecting secondary structural elements. The remaining amino acid substitutions are distributed throughout the sequence and are not predicted to alter the polypeptide fold. Parafusin contains a putative protein kinase C site located on a surface loop in domain II that is not present in the homologs. Although the general domain structure and the active site of rabbit muscle phosphoglucomutase are preserved in the model of phosphoglucomutase-related protein, a major structural difference is likely to occur in domain 1 due to the absence of 55 amino acid residues in PGM-RP. This deletion predicts the loss of three α-helices and one β-strand from an anti-parallel β-sheet in this domain as compared with the rabbit muscle phosphoglucomutase.

Original languageEnglish (US)
Pages (from-to)737-746
Number of pages10
JournalProtein Engineering
Volume12
Issue number9
StatePublished - 1999

Fingerprint

Phosphoglucomutase
Muscle
Proteins
Amino acids
Polypeptides
Rabbits
Substitution reactions
Muscles
Crystal structure
Sarcoplasmic Reticulum
Amino Acids
Structural Models
Amino Acid Substitution
Protein Kinase C

Keywords

  • Aciculin
  • Parafusin
  • Phosphoglucomutase
  • Protein superfamily

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Functional diversity of the phosphoglucomutase superfamily : Structural implications. / Levin, Sergei; Almo, Steven C.; Satir, Birgit H.

In: Protein Engineering, Vol. 12, No. 9, 1999, p. 737-746.

Research output: Contribution to journalArticle

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