Functional coordination of three mitotic motors in Drosophila embryos

David J. Sharp, Heather M. Brown, Mijung Kwon, Gregory C. Rogers, Gina Holland, Jonathan M. Scholey

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

It is well established that multiple microtubule-based motors contribute to the formation and function of the mitotic spindle, but how the activities of these motors interrelate remains unclear. Here we visualize spindle formation in living Drosophila embryos to show that spindle pole movements are directed by a temporally coordinated balance of forces generated by three mitotic motors, cytoplasmic dynein, KLP61F, and Ncd. Specifically, our findings suggest that dynein acts to move the poles apart throughout mitosis and that this activity is augmented by KLP61F after the fenestration of the nuclear envelope, a process analogous to nuclear envelope breakdown, which occurs at the onset of prometaphase. Conversely, we find that Ncd generates forces that pull the poles together between interphase and metaphase, antagonizing the activity of both dynein and KLP61F and serving as a brake for spindle assembly. During anaphase, however, Ncd appears to have no effect on spindle pole movements, suggesting that its activity is downregulated at this time, allowing dynein and KLP61F to drive spindle elongation during anaphase B.

Original languageEnglish (US)
Pages (from-to)241-253
Number of pages13
JournalMolecular Biology of the Cell
Volume11
Issue number1
StatePublished - Jan 2000
Externally publishedYes

Fingerprint

Dyneins
Spindle Poles
Drosophila
Anaphase
Embryonic Structures
Nuclear Envelope
Cytoplasmic Dyneins
Prometaphase
Spindle Apparatus
Interphase
Metaphase
Mitosis
Microtubules
Motor Activity
Down-Regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Sharp, D. J., Brown, H. M., Kwon, M., Rogers, G. C., Holland, G., & Scholey, J. M. (2000). Functional coordination of three mitotic motors in Drosophila embryos. Molecular Biology of the Cell, 11(1), 241-253.

Functional coordination of three mitotic motors in Drosophila embryos. / Sharp, David J.; Brown, Heather M.; Kwon, Mijung; Rogers, Gregory C.; Holland, Gina; Scholey, Jonathan M.

In: Molecular Biology of the Cell, Vol. 11, No. 1, 01.2000, p. 241-253.

Research output: Contribution to journalArticle

Sharp, DJ, Brown, HM, Kwon, M, Rogers, GC, Holland, G & Scholey, JM 2000, 'Functional coordination of three mitotic motors in Drosophila embryos', Molecular Biology of the Cell, vol. 11, no. 1, pp. 241-253.
Sharp DJ, Brown HM, Kwon M, Rogers GC, Holland G, Scholey JM. Functional coordination of three mitotic motors in Drosophila embryos. Molecular Biology of the Cell. 2000 Jan;11(1):241-253.
Sharp, David J. ; Brown, Heather M. ; Kwon, Mijung ; Rogers, Gregory C. ; Holland, Gina ; Scholey, Jonathan M. / Functional coordination of three mitotic motors in Drosophila embryos. In: Molecular Biology of the Cell. 2000 ; Vol. 11, No. 1. pp. 241-253.
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