Functional cloning of genes regulating apoptosis in neuronal cells.

Roberta Visconti, Luciano D'Adamio

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Here, we describe the use of a functional cloning approach, based on the screen of a genome-wide short hairpin RNA (shRNA) library, to identify novel genes regulating apoptosis in neuronal cells. Apoptosis is induced by doxorubicin and is detected with a fluorometric caspase 3 assay. Moreover, we describe also the screen of the library to identify genes regulating the processing f the beta-amyloid (Abeta) precursor protein (APP), the protein associated with the pathogenesis of lzheimer's disease. The levels of the peptide Abeta, produced by the APP processing, are detected with ELISA based on the innovative Delfia method developed by PerkinElmer.

Original languageEnglish (US)
Pages (from-to)125-131
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Volume399
StatePublished - 2007
Externally publishedYes

Fingerprint

Organism Cloning
Apoptosis
Protein Precursors
Amyloid beta-Protein Precursor
Caspase 3
Doxorubicin
Small Interfering RNA
Genes
Libraries
Enzyme-Linked Immunosorbent Assay
Genome
Peptides
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Functional cloning of genes regulating apoptosis in neuronal cells. / Visconti, Roberta; D'Adamio, Luciano.

In: Methods in molecular biology (Clifton, N.J.), Vol. 399, 2007, p. 125-131.

Research output: Contribution to journalArticle

Visconti, Roberta ; D'Adamio, Luciano. / Functional cloning of genes regulating apoptosis in neuronal cells. In: Methods in molecular biology (Clifton, N.J.). 2007 ; Vol. 399. pp. 125-131.
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