Functional characterization of an insulin-responsive glucose transporter (GLUT4) from fish adipose tissue

Encarnación Capilla, Mònica Díaz, Amaya Albalat, Isabel Navarro, Jeffrey E. Pessin, Konrad Keller, Josep V. Planas

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Glucose transport across the plasma membrane is mediated by a family of glucose transporter proteins (GLUTs), several of which have been identified in mammalian, avian, and, more recently, in fish species. Here, we report on the cloning of a salmon GLUT from adipose tissue with a high sequence homology to mammalian GLUT4 that has been named okGLUT4. Kinetic analysis of glucose transport following expression in Xenopus laevis oocytes demonstrated a 7.6 ± 1.4 mM Km for 2-deoxyglucose (2-DG) transport measured under zero-trans conditions and 14.4 ± 1.5 mM by equilibrium exchange of 3-O-methylglucose. Transport of 2-DG by okGLUT4-injected oocytes was stereospecific and was competed by D-glucose, D-mannose, and, to a lesser extent, D-galactose and D-fructose. In addition, 2-DG uptake was inhibited by cytochalasin B and ethylidene glucose. Moreover, insulin stimulated glucose uptake in Xenopus oocytes expressing okGLUT4 and in isolated trout adipocytes, which contain the native form of okGLUT4. Despite differences in protein motifs important for insulin-stimulated translocation of mammalian GLUT4, okGLUT4 was able to translocate to the plasma membrane from intracellular localization sites in response to insulin when expressed in 3T3-L1 adipocytes. These data demonstrate that okGLUT4 is a structural and functional fish homolog of mammalian GLUT4 but with a lower affinity for glucose, which could in part explain the lower ability of fish to clear a glucose load.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume287
Issue number2 50-2
DOIs
StatePublished - Aug 2004
Externally publishedYes

Fingerprint

Glucose Transporter Type 4
Fish
Adipose Tissue
Fishes
Tissue
Glucose
Deoxyglucose
Oocytes
Facilitative Glucose Transport Proteins
Cell membranes
Insulin
Adipocytes
Cell Membrane
3-O-Methylglucose
Amino Acid Motifs
Cytochalasin B
Trout
Salmon
Cloning
Xenopus laevis

Keywords

  • Glucose uptake
  • Immunolocalization
  • Xenopus oocytes

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Functional characterization of an insulin-responsive glucose transporter (GLUT4) from fish adipose tissue. / Capilla, Encarnación; Díaz, Mònica; Albalat, Amaya; Navarro, Isabel; Pessin, Jeffrey E.; Keller, Konrad; Planas, Josep V.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 287, No. 2 50-2, 08.2004.

Research output: Contribution to journalArticle

Capilla, Encarnación ; Díaz, Mònica ; Albalat, Amaya ; Navarro, Isabel ; Pessin, Jeffrey E. ; Keller, Konrad ; Planas, Josep V. / Functional characterization of an insulin-responsive glucose transporter (GLUT4) from fish adipose tissue. In: American Journal of Physiology - Endocrinology and Metabolism. 2004 ; Vol. 287, No. 2 50-2.
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