Functional analysis of the fibrinogen-related scabrous gene from Drosophila melanogaster identifies potential effector and stimulatory protein domains

E. Chiang Lee, Sung Yun Yu, Xiaoxi Hu, Marek Mlodzik, Nicholas E. Baker

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16 Citations (Scopus)

Abstract

The scabrous (sca) gene encodes a secreted dimeric glycoprotein with putative coiled-coil domains N-terminally and a C-terminal region related to the blood clot protein fibrinogen. Homozygous sca mutants have extra bristle organs and rough eyes. We describe a GAL4-based expression system for testing rescue of the sca mutant phenotype by altered SCA proteins and for misexpression. We find that deletion of the fibrinogen-related domain (FRED) greatly decreases SCA function, confirming the importance of this conserved region. SCA function could not be restored by FReDs from human fibrinogen chain genes However, proteins lacking any FReD still showed some function in both rescue and misexpression experiments, suggesting that putative effector- binding regions lie outside this domain. Consistent with this, proteins expressing only the FReD had no rescuing activity but were recessive negative; i.e., they enhanced the phenotype of sca mutations but had no phenotype in the presence of a wild-type sea allele. This suggests that the FReD contributes to SCA function by binding to other components of the bristle determination pathway, increasing the activity of the linked N- terminal region.

Original languageEnglish (US)
Pages (from-to)663-673
Number of pages11
JournalGenetics
Volume150
Issue number2
StatePublished - 1998

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Drosophila melanogaster
Fibrinogen
Phenotype
Genes
Proteins
Oceans and Seas
Blood Proteins
Glycoproteins
Thrombosis
Alleles
Mutation
Protein Domains

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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Functional analysis of the fibrinogen-related scabrous gene from Drosophila melanogaster identifies potential effector and stimulatory protein domains. / Lee, E. Chiang; Yu, Sung Yun; Hu, Xiaoxi; Mlodzik, Marek; Baker, Nicholas E.

In: Genetics, Vol. 150, No. 2, 1998, p. 663-673.

Research output: Contribution to journalArticle

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AU - Mlodzik, Marek

AU - Baker, Nicholas E.

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