Function of oxidative stress in the regulation of hematopoietic stem cell-niche interaction

Kentaro Hosokawa; Fumio Arai; Hiroki Yoshihara; Yuka Nakamura; Yumiko Gomei; Hiroko Iwasaki; Kana Miyamoto; Haruko Shima; Keisuke Ito; Toshio Suda

doi:10.1016/j.bbrc.2007.09.014

Function of oxidative stress in the regulation of hematopoietic stem cell-niche interaction

Kentaro Hosokawa, Fumio Arai, Hiroki Yoshihara, Yuka Nakamura, Yumiko Gomei, Hiroko Iwasaki, Kana Miyamoto, Haruko Shima, Keisuke Ito, Toshio Suda

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

During postnatal life, the bone marrow (BM) supports both self-renewal and differentiation of hematopoietic stem cells (HSCs) in specialized niches, such as osteoblastic niche and vascular niche. A cell adhesion molecule, N-cadherin expressed in the HSCs and osteoblasts, suggesting that homophylic binding of N-cadherin induce the adhesion of HSCs to the niche cells. Here we demonstrate that an anti-cancer drug, 5-fuluorouracil induces reactive oxygen species (ROS) in HSCs, which suppressed N-cadherin expression. These events result in the shift of side population (SP) cells to non-SP cells, indicating that quiescent HSCs are detached from the niche. Administration of a potent anti-oxidant, N-acetyl cystein (NAC) suppressed the shift from SP cells. These data suggest that ROS suppressed the N-cadherin-mediated cell adhesion, and induce the exit of HSCs from the niche.

Original language	English (US)
Pages (from-to)	578-583
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	363
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2007.09.014
State	Published - Nov 23 2007
Externally published	Yes

Keywords

Cell adhesion
Cell cycle
Hematopoietic stem cell
N-cadherin
Niche
Oxidative stress

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2007.09.014

Cite this

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title = "Function of oxidative stress in the regulation of hematopoietic stem cell-niche interaction",

abstract = "During postnatal life, the bone marrow (BM) supports both self-renewal and differentiation of hematopoietic stem cells (HSCs) in specialized niches, such as osteoblastic niche and vascular niche. A cell adhesion molecule, N-cadherin expressed in the HSCs and osteoblasts, suggesting that homophylic binding of N-cadherin induce the adhesion of HSCs to the niche cells. Here we demonstrate that an anti-cancer drug, 5-fuluorouracil induces reactive oxygen species (ROS) in HSCs, which suppressed N-cadherin expression. These events result in the shift of side population (SP) cells to non-SP cells, indicating that quiescent HSCs are detached from the niche. Administration of a potent anti-oxidant, N-acetyl cystein (NAC) suppressed the shift from SP cells. These data suggest that ROS suppressed the N-cadherin-mediated cell adhesion, and induce the exit of HSCs from the niche.",

keywords = "Cell adhesion, Cell cycle, Hematopoietic stem cell, N-cadherin, Niche, Oxidative stress",

author = "Kentaro Hosokawa and Fumio Arai and Hiroki Yoshihara and Yuka Nakamura and Yumiko Gomei and Hiroko Iwasaki and Kana Miyamoto and Haruko Shima and Keisuke Ito and Toshio Suda",

note = "Funding Information: We also thank Ayako Kumakubo and Ayami Ono for technical assistance. This work was supported by a Grant-in-Aid for Specially Promoted Research and a Grant-in-Aid for Young Scientists (A) from The Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and by the“High-Tech Research Center” Project for Private Universities: matching fund subsidy from MEXT. ",

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doi = "10.1016/j.bbrc.2007.09.014",

language = "English (US)",

volume = "363",

pages = "578--583",

journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - Function of oxidative stress in the regulation of hematopoietic stem cell-niche interaction

AU - Hosokawa, Kentaro

AU - Arai, Fumio

AU - Yoshihara, Hiroki

AU - Nakamura, Yuka

AU - Gomei, Yumiko

AU - Iwasaki, Hiroko

AU - Miyamoto, Kana

AU - Shima, Haruko

AU - Ito, Keisuke

AU - Suda, Toshio

N1 - Funding Information: We also thank Ayako Kumakubo and Ayami Ono for technical assistance. This work was supported by a Grant-in-Aid for Specially Promoted Research and a Grant-in-Aid for Young Scientists (A) from The Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and by the“High-Tech Research Center” Project for Private Universities: matching fund subsidy from MEXT.

PY - 2007/11/23

Y1 - 2007/11/23

N2 - During postnatal life, the bone marrow (BM) supports both self-renewal and differentiation of hematopoietic stem cells (HSCs) in specialized niches, such as osteoblastic niche and vascular niche. A cell adhesion molecule, N-cadherin expressed in the HSCs and osteoblasts, suggesting that homophylic binding of N-cadherin induce the adhesion of HSCs to the niche cells. Here we demonstrate that an anti-cancer drug, 5-fuluorouracil induces reactive oxygen species (ROS) in HSCs, which suppressed N-cadherin expression. These events result in the shift of side population (SP) cells to non-SP cells, indicating that quiescent HSCs are detached from the niche. Administration of a potent anti-oxidant, N-acetyl cystein (NAC) suppressed the shift from SP cells. These data suggest that ROS suppressed the N-cadherin-mediated cell adhesion, and induce the exit of HSCs from the niche.

AB - During postnatal life, the bone marrow (BM) supports both self-renewal and differentiation of hematopoietic stem cells (HSCs) in specialized niches, such as osteoblastic niche and vascular niche. A cell adhesion molecule, N-cadherin expressed in the HSCs and osteoblasts, suggesting that homophylic binding of N-cadherin induce the adhesion of HSCs to the niche cells. Here we demonstrate that an anti-cancer drug, 5-fuluorouracil induces reactive oxygen species (ROS) in HSCs, which suppressed N-cadherin expression. These events result in the shift of side population (SP) cells to non-SP cells, indicating that quiescent HSCs are detached from the niche. Administration of a potent anti-oxidant, N-acetyl cystein (NAC) suppressed the shift from SP cells. These data suggest that ROS suppressed the N-cadherin-mediated cell adhesion, and induce the exit of HSCs from the niche.

KW - Cell adhesion

KW - Cell cycle

KW - Hematopoietic stem cell

KW - N-cadherin

KW - Niche

KW - Oxidative stress

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Function of oxidative stress in the regulation of hematopoietic stem cell-niche interaction

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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