Function of oxidative stress in the regulation of hematopoietic stem cell-niche interaction

Kentaro Hosokawa, Fumio Arai, Hiroki Yoshihara, Yuka Nakamura, Yumiko Gomei, Hiroko Iwasaki, Kana Miyamoto, Haruko Shima, Keisuke Ito, Toshio Suda

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

During postnatal life, the bone marrow (BM) supports both self-renewal and differentiation of hematopoietic stem cells (HSCs) in specialized niches, such as osteoblastic niche and vascular niche. A cell adhesion molecule, N-cadherin expressed in the HSCs and osteoblasts, suggesting that homophylic binding of N-cadherin induce the adhesion of HSCs to the niche cells. Here we demonstrate that an anti-cancer drug, 5-fuluorouracil induces reactive oxygen species (ROS) in HSCs, which suppressed N-cadherin expression. These events result in the shift of side population (SP) cells to non-SP cells, indicating that quiescent HSCs are detached from the niche. Administration of a potent anti-oxidant, N-acetyl cystein (NAC) suppressed the shift from SP cells. These data suggest that ROS suppressed the N-cadherin-mediated cell adhesion, and induce the exit of HSCs from the niche.

Original languageEnglish (US)
Pages (from-to)578-583
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume363
Issue number3
DOIs
StatePublished - Nov 23 2007
Externally publishedYes

Fingerprint

Stem Cell Niche
Oxidative stress
Hematopoietic Stem Cells
Stem cells
Cell Communication
Oxidative Stress
Cadherins
Side-Population Cells
Reactive Oxygen Species
Osteoblasts
Cell adhesion
Cell Adhesion Molecules
Oxidants
Cell Adhesion
Blood Vessels
Bone
Adhesion
Bone Marrow
Pharmaceutical Preparations
Population

Keywords

  • Cell adhesion
  • Cell cycle
  • Hematopoietic stem cell
  • N-cadherin
  • Niche
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Function of oxidative stress in the regulation of hematopoietic stem cell-niche interaction. / Hosokawa, Kentaro; Arai, Fumio; Yoshihara, Hiroki; Nakamura, Yuka; Gomei, Yumiko; Iwasaki, Hiroko; Miyamoto, Kana; Shima, Haruko; Ito, Keisuke; Suda, Toshio.

In: Biochemical and Biophysical Research Communications, Vol. 363, No. 3, 23.11.2007, p. 578-583.

Research output: Contribution to journalArticle

Hosokawa, K, Arai, F, Yoshihara, H, Nakamura, Y, Gomei, Y, Iwasaki, H, Miyamoto, K, Shima, H, Ito, K & Suda, T 2007, 'Function of oxidative stress in the regulation of hematopoietic stem cell-niche interaction', Biochemical and Biophysical Research Communications, vol. 363, no. 3, pp. 578-583. https://doi.org/10.1016/j.bbrc.2007.09.014
Hosokawa, Kentaro ; Arai, Fumio ; Yoshihara, Hiroki ; Nakamura, Yuka ; Gomei, Yumiko ; Iwasaki, Hiroko ; Miyamoto, Kana ; Shima, Haruko ; Ito, Keisuke ; Suda, Toshio. / Function of oxidative stress in the regulation of hematopoietic stem cell-niche interaction. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 363, No. 3. pp. 578-583.
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AU - Iwasaki, Hiroko

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