Frizzled-Dishevelled signaling specificity outcome can be modulated by Diego in Drosophila

Jun Wu, Andreas Jenny, Ivana Mirkovic, Marek Mlodzik

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Members of the Frizzled (Fz) family of seven-pass transmembrane receptors are required for the transduction of both Wnt-Fz/β-catenin and Fz/planar cell polarity (PCP) signals. Although both pathways transduce signals via interactions between Fz and the cytoplasmic protein Dishevelled (Dsh), each pathway has specific and distinct effectors. One explanation for the pathway specificity is that signal-induced conformational changes result in unique Fz-Dsh interactions. Our mutational analyses of Fz-Dsh activities in vivo do however not support this model, since both pathways are affected by all mutations tested. Alternatively, the interaction of Fz or Dsh with other proteins could modulate the signaling outcome. We examined the role of a Dsh-binding PCP molecule, Diego (Dgo), in both Wnt-Fz/β-catenin and Fz/PCP signaling. Both loss-of-function and gain-of-function results suggest that Dgo promotes Fz-Dsh/PCP signaling at the expense of Wnt-Fz/β-catenin signaling. Our data suggest that Dgo sequesters Dsh to a functionally distinct Fz/PCP signaling compartment within the cell.

Original languageEnglish (US)
Pages (from-to)30-42
Number of pages13
JournalMechanisms of Development
Volume125
Issue number1-2
DOIs
StatePublished - Jan 2008
Externally publishedYes

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Cell Polarity
Drosophila
Catenins
Frizzled Receptors
Signal Transduction
Mutation
Proteins

Keywords

  • Fz-signaling
  • Patterning
  • Planar cell polarity
  • Protein interactions
  • Signaling specificity

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Frizzled-Dishevelled signaling specificity outcome can be modulated by Diego in Drosophila. / Wu, Jun; Jenny, Andreas; Mirkovic, Ivana; Mlodzik, Marek.

In: Mechanisms of Development, Vol. 125, No. 1-2, 01.2008, p. 30-42.

Research output: Contribution to journalArticle

Wu, Jun ; Jenny, Andreas ; Mirkovic, Ivana ; Mlodzik, Marek. / Frizzled-Dishevelled signaling specificity outcome can be modulated by Diego in Drosophila. In: Mechanisms of Development. 2008 ; Vol. 125, No. 1-2. pp. 30-42.
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