Frequent Amplification and Rearrangement of Chromosomal Bands 6p12-p21 and 17p11.2 in Osteosarcoma

Ching C. Lau, Charles P. Harris, Xin Yan Lu, Laszlo Perlaky, Sheila Gogineni, Murali Chintagumpala, John Hicks, Mark E. Johnson, Nelson A. Davino, Andrew G. Huvos, Paul A. Meyers, John H. Healy, Richard Gorlick, Pulivarthi H. Rao

Research output: Contribution to journalArticlepeer-review

114 Scopus citations

Abstract

Osteosarcoma (OS) is a highly malignant bone neoplasm of children and young adults. It is characterized by chaotic karyotypes with complex marker chromosomes. We applied a combination of molecular cytogenetic techniques including comparative genomic hybridization (CGH), spectral karyotyping (SKY), and fluorescence in situ hybridization (FISH) to decipher the chromosomal complexity in a panel of 25 tumors. Combined SKY and G-banding analysis identified several novel recurrent breakpoint clusters and 9 nonrecurrent reciprocal translocations. CGH identified several recurrent chromosomal losses including 2q, 3p, 9, 10p, 12q, 13q, 14q, 15q, 16, 17p, and 18q, gains including Xp, Xq, 5q, 6p, 8q, 17p, and 20q, and high-level chromosomal amplifications at Xp11.2, 1q21-q22, 4p11, 4q12, 5p15, 6p12.1, 8q13, 8q23, 10q11, 10q22, 11q13, 11q23, 12q13-q14, 13q21-q34, 16q22, 17p11.2, 17q21-q22, 18q22, 20p11.2, and 20q12. Frequent amplification and rearrangement involving chromosomal bands at 6p12-p21 and 17p11.2 were found in 28% and 32% of cases, respectively. In an attempt to identify the genes involved in these amplicons, we used three nonoverlapping BAC clones contained within each amplicon as probes for FISH analysis, leading to a more detailed characterization and quantification of the 6p and 17p amplicons.

Original languageEnglish (US)
Pages (from-to)11-21
Number of pages11
JournalGenes Chromosomes and Cancer
Volume39
Issue number1
DOIs
StatePublished - Jan 1 2004

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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