Free radical generation by early glycation products: A mechanism for accelerated atherogenesis in diabetes

Cathleen J. Mullarkey, Diane Edelstein, Michael Brownlee

Research output: Contribution to journalArticle

612 Scopus citations

Abstract

Non-enzymatic glycation of reactive amino groups in model proteins increased the rate of free radical production at physiologic pH by nearly fifty-fold over non-glycated protein. Superoxide generation was confirmed by electron paramagnetic resonance measurements with the spin-trap phenyl-t-butyl-nitrone. Both Schiff base and Amadori glycation products were found to generate free radicals in a ratio of 1:1.5. Free radicals generated by glycated protein increased peroxidation of membranes of linoleic/arachidonic acid vesicles nearly 2-fold over control, suggesting that the increased glycation of proteins in diabetes may accelerate vascular wall lipid oxidative modification.

Original languageEnglish (US)
Pages (from-to)932-939
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume173
Issue number3
DOIs
StatePublished - Dec 31 1990

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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