TY - JOUR
T1 - Free radical generation by early glycation products
T2 - A mechanism for accelerated atherogenesis in diabetes
AU - Mullarkey, Cathleen J.
AU - Edelstein, Diane
AU - Brownlee, Michael
N1 - Funding Information:
This work was supported by National Institute Diseases (NIDDK) grants DK33861 and DK07004.
PY - 1990/12/31
Y1 - 1990/12/31
N2 - Non-enzymatic glycation of reactive amino groups in model proteins increased the rate of free radical production at physiologic pH by nearly fifty-fold over non-glycated protein. Superoxide generation was confirmed by electron paramagnetic resonance measurements with the spin-trap phenyl-t-butyl-nitrone. Both Schiff base and Amadori glycation products were found to generate free radicals in a ratio of 1:1.5. Free radicals generated by glycated protein increased peroxidation of membranes of linoleic/arachidonic acid vesicles nearly 2-fold over control, suggesting that the increased glycation of proteins in diabetes may accelerate vascular wall lipid oxidative modification.
AB - Non-enzymatic glycation of reactive amino groups in model proteins increased the rate of free radical production at physiologic pH by nearly fifty-fold over non-glycated protein. Superoxide generation was confirmed by electron paramagnetic resonance measurements with the spin-trap phenyl-t-butyl-nitrone. Both Schiff base and Amadori glycation products were found to generate free radicals in a ratio of 1:1.5. Free radicals generated by glycated protein increased peroxidation of membranes of linoleic/arachidonic acid vesicles nearly 2-fold over control, suggesting that the increased glycation of proteins in diabetes may accelerate vascular wall lipid oxidative modification.
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U2 - 10.1016/S0006-291X(05)80875-7
DO - 10.1016/S0006-291X(05)80875-7
M3 - Article
C2 - 2176495
AN - SCOPUS:0025540553
SN - 0006-291X
VL - 173
SP - 932
EP - 939
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
