Fragmentation of the Golgi apparatus of motor neurons in amyotrophic lateral sclerosis revealed by organelle-specific antibodies

Z. Mourelatos, H. Adler, A. Hirano, H. Donnenfeld, J. O. Gonatas, N. K. Gonatas

Research output: Contribution to journalArticle

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Abstract

Many studies have established the central involvement of the Golgi apparatus in the transport and processing of plasma membrane, lysosomal, and secreted proteins. The Golgi apparatus of neurons is also involved in the neoplasmic flow of fast-moving macromolecules and in the arthograde, retrograde, and transsynaptic transport of exogenous ligands. Markers of the Golgi apparatus, based on traditional methods of enzyme cytochemistry, are not applicable to human tissues obtained at autopsy. For that reason, the Golgi apparatus of brain cells has not been examined adequately in diseases of the human nervous system. Here we report that an antiserum raised against MG-160, a 160-kDa sialoglycoprotein of medial cisternae of the Golgi apparatus of several rats cells, is a specific and easily reproducible immunocytochemical marker of the Golgi apparatus of human neurons and other cells obtained at autopsy. Application of this probe in amyotrophic lateral sclerosis has shown a fragmentation of the Golgi apparatus in motor neurons similar to that induced by depolymerization of microtubules. We suggest that the fragmentation of the Golgi apparatus of motor neurons in amyotrophic lateral sclerosis has functional implications because significant reductions of secretion of insulin and immunoglobulins have been observed in islet cells and plasma cells, respectively, treated with microtubule-disrupting agents.

Original languageEnglish (US)
Pages (from-to)4393-4395
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number11
StatePublished - 1990

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Amyotrophic Lateral Sclerosis
Golgi Apparatus
Motor Neurons
Organelles
Antibodies
Microtubules
Autopsy
Sialoglycoproteins
Neurons
Histocytochemistry
Plasma Cells
Nervous System Diseases
Islets of Langerhans
Immunoglobulins
Immune Sera
Cell Membrane
Insulin
Ligands
Brain
Enzymes

Keywords

  • Immunoytochemistry
  • Membrane polypeptide
  • Morphometry

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Fragmentation of the Golgi apparatus of motor neurons in amyotrophic lateral sclerosis revealed by organelle-specific antibodies. / Mourelatos, Z.; Adler, H.; Hirano, A.; Donnenfeld, H.; Gonatas, J. O.; Gonatas, N. K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 11, 1990, p. 4393-4395.

Research output: Contribution to journalArticle

Mourelatos, Z. ; Adler, H. ; Hirano, A. ; Donnenfeld, H. ; Gonatas, J. O. ; Gonatas, N. K. / Fragmentation of the Golgi apparatus of motor neurons in amyotrophic lateral sclerosis revealed by organelle-specific antibodies. In: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Vol. 87, No. 11. pp. 4393-4395.
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