Fractions of HeLa DNA differing in their content of guanine + cytosine

Carl L. Schildkraut, Joseph J. Maio

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C contents according to the relation: ρ{variant} = 0.98 (G + C) + 1.659, where ρ{variant} refers to buoyant density and (G + C) to the average mole fraction of guanine + cytosine. This nucleolar DNA is significantly enriched with respect to two satellite bands: a light satellite (ρ{variant} = 1.686 g/ml.) and a heavy satellite (ρ{variant} = 1.712 g/ml.). In an alkaline CsCl density gradient, the light satellite DNA can be separated into two complementary strands, as demonstrated by base composition analysis.

Original languageEnglish (US)
Pages (from-to)305-312
Number of pages8
JournalJournal of Molecular Biology
Volume46
Issue number2
DOIs
StatePublished - Dec 14 1969

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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