Fractions of HeLa DNA differing in their content of guanine + cytosine

Carl L. Schildkraut, Joseph J. Maio

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C contents according to the relation: ρ{variant} = 0.98 (G + C) + 1.659, where ρ{variant} refers to buoyant density and (G + C) to the average mole fraction of guanine + cytosine. This nucleolar DNA is significantly enriched with respect to two satellite bands: a light satellite (ρ{variant} = 1.686 g/ml.) and a heavy satellite (ρ{variant} = 1.712 g/ml.). In an alkaline CsCl density gradient, the light satellite DNA can be separated into two complementary strands, as demonstrated by base composition analysis.

Original languageEnglish (US)
Pages (from-to)305-312
Number of pages8
JournalJournal of Molecular Biology
Volume46
Issue number2
StatePublished - Dec 14 1969

Fingerprint

Base Composition
Cell Nucleolus
Satellite DNA
Light
Cytosine
DNA
Guanine
HeLa Cells
cesium chloride

ASJC Scopus subject areas

  • Virology

Cite this

Fractions of HeLa DNA differing in their content of guanine + cytosine. / Schildkraut, Carl L.; Maio, Joseph J.

In: Journal of Molecular Biology, Vol. 46, No. 2, 14.12.1969, p. 305-312.

Research output: Contribution to journalArticle

@article{5bee3b33d14743a98514ae5b47438c52,
title = "Fractions of HeLa DNA differing in their content of guanine + cytosine",
abstract = "DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C contents according to the relation: ρ{variant} = 0.98 (G + C) + 1.659, where ρ{variant} refers to buoyant density and (G + C) to the average mole fraction of guanine + cytosine. This nucleolar DNA is significantly enriched with respect to two satellite bands: a light satellite (ρ{variant} = 1.686 g/ml.) and a heavy satellite (ρ{variant} = 1.712 g/ml.). In an alkaline CsCl density gradient, the light satellite DNA can be separated into two complementary strands, as demonstrated by base composition analysis.",
author = "Schildkraut, {Carl L.} and Maio, {Joseph J.}",
year = "1969",
month = "12",
day = "14",
language = "English (US)",
volume = "46",
pages = "305--312",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Fractions of HeLa DNA differing in their content of guanine + cytosine

AU - Schildkraut, Carl L.

AU - Maio, Joseph J.

PY - 1969/12/14

Y1 - 1969/12/14

N2 - DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C contents according to the relation: ρ{variant} = 0.98 (G + C) + 1.659, where ρ{variant} refers to buoyant density and (G + C) to the average mole fraction of guanine + cytosine. This nucleolar DNA is significantly enriched with respect to two satellite bands: a light satellite (ρ{variant} = 1.686 g/ml.) and a heavy satellite (ρ{variant} = 1.712 g/ml.). In an alkaline CsCl density gradient, the light satellite DNA can be separated into two complementary strands, as demonstrated by base composition analysis.

AB - DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C contents according to the relation: ρ{variant} = 0.98 (G + C) + 1.659, where ρ{variant} refers to buoyant density and (G + C) to the average mole fraction of guanine + cytosine. This nucleolar DNA is significantly enriched with respect to two satellite bands: a light satellite (ρ{variant} = 1.686 g/ml.) and a heavy satellite (ρ{variant} = 1.712 g/ml.). In an alkaline CsCl density gradient, the light satellite DNA can be separated into two complementary strands, as demonstrated by base composition analysis.

UR - http://www.scopus.com/inward/record.url?scp=0014675197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014675197&partnerID=8YFLogxK

M3 - Article

C2 - 5360041

AN - SCOPUS:0014675197

VL - 46

SP - 305

EP - 312

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 2

ER -