TY - JOUR
T1 - Fractions of HeLa DNA differing in their content of guanine + cytosine
AU - Schildkraut, Carl L.
AU - Maio, Joseph J.
N1 - Funding Information:
We are grateful to Dr Elliott Levine for his collaboration in the experiments with M~co~laema, and to Mr Eric Brown and Mr David Kurnit for their assistance. These studies were supported by the National Institutes of Health (AI 4153), the National Science Foundaton, and the American Cancer Society. One of us (C.L.S.) is a Kennedy Scholar.
PY - 1969/12/14
Y1 - 1969/12/14
N2 - DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C contents according to the relation: ρ{variant} = 0.98 (G + C) + 1.659, where ρ{variant} refers to buoyant density and (G + C) to the average mole fraction of guanine + cytosine. This nucleolar DNA is significantly enriched with respect to two satellite bands: a light satellite (ρ{variant} = 1.686 g/ml.) and a heavy satellite (ρ{variant} = 1.712 g/ml.). In an alkaline CsCl density gradient, the light satellite DNA can be separated into two complementary strands, as demonstrated by base composition analysis.
AB - DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C contents according to the relation: ρ{variant} = 0.98 (G + C) + 1.659, where ρ{variant} refers to buoyant density and (G + C) to the average mole fraction of guanine + cytosine. This nucleolar DNA is significantly enriched with respect to two satellite bands: a light satellite (ρ{variant} = 1.686 g/ml.) and a heavy satellite (ρ{variant} = 1.712 g/ml.). In an alkaline CsCl density gradient, the light satellite DNA can be separated into two complementary strands, as demonstrated by base composition analysis.
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U2 - 10.1016/0022-2836(69)90423-9
DO - 10.1016/0022-2836(69)90423-9
M3 - Article
C2 - 5360041
AN - SCOPUS:0014675197
SN - 0022-2836
VL - 46
SP - 305
EP - 312
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 2
ER -