FOXP3 inhibits activation-induced NFAT2 expression in T cells thereby limiting effector cytokine expression

Troy R. Torgerson, Anna Genin, Chunxia Chen, Mingce Zhang, Bin Zhou, Stephanie Añover-Sombke, M. Barton Frank, Igor Dozmorov, Elizabeth Ocheltree, Petri Kulmala, Michael Centola, Hans D. Ochs, Andrew D. Wells, Randy Q. Cron

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The forkhead DNA-binding protein FOXP3 is critical for the development and suppressive function of CD4+CD25+ regulatory T cells (TREG), which play a key role in maintaining self-tolerance. Functionally, FOXP3 is capable of repressing transcription of cytokine genes regulated by NFAT. Various mechanisms have been proposed by which FOXP3 mediates these effects. Using novel cell lines that inducibly express either wild-type or mutant FOXP3, we have identified NFAT2 as an early target of FOXP3-mediated transcriptional repression. NFAT2 is typically expressed at low levels in resting T cells, but is up-regulated by NFAT1 upon cellular activation. We demonstrate that transcription from the NFAT2 promoter is significantly suppressed by FOXP3, and NFAT2 protein expression is markedly diminished in activated CD4+CD25+FOXP3+ TREG compared with CD4+CD25-FOXP3- T cells. Chromatin immunoprecipitation experiments indicate that FOXP3 competes with NFAT1 for binding to the endogenous NFAT2 promoter. This antagonism of NFAT2 activity by FOXP3 is important for the anergic phenotype of TREG, as ectopic expression of NFAT2 from a retroviral LTR partially restores expression of IL-2 in FOXP3+ TREG. These data suggest that FOXP3 functions not only to suppress the first wave of NFAT-mediated transcriptional responses, but may also affect sustained NFAT-mediated inflammatory gene expression through suppression of inducible NFAT2 transcription.

Original languageEnglish (US)
Pages (from-to)907-915
Number of pages9
JournalJournal of Immunology
Volume183
Issue number2
DOIs
StatePublished - Jul 15 2009

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Cytokines
NFATC Transcription Factors
Forkhead Transcription Factors
T-Lymphocytes
Self Tolerance
Chromatin Immunoprecipitation
DNA-Binding Proteins
Regulatory T-Lymphocytes
Interleukin-2
Phenotype
Gene Expression
Cell Line
Genes
Ectopic Gene Expression

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

FOXP3 inhibits activation-induced NFAT2 expression in T cells thereby limiting effector cytokine expression. / Torgerson, Troy R.; Genin, Anna; Chen, Chunxia; Zhang, Mingce; Zhou, Bin; Añover-Sombke, Stephanie; Frank, M. Barton; Dozmorov, Igor; Ocheltree, Elizabeth; Kulmala, Petri; Centola, Michael; Ochs, Hans D.; Wells, Andrew D.; Cron, Randy Q.

In: Journal of Immunology, Vol. 183, No. 2, 15.07.2009, p. 907-915.

Research output: Contribution to journalArticle

Torgerson, TR, Genin, A, Chen, C, Zhang, M, Zhou, B, Añover-Sombke, S, Frank, MB, Dozmorov, I, Ocheltree, E, Kulmala, P, Centola, M, Ochs, HD, Wells, AD & Cron, RQ 2009, 'FOXP3 inhibits activation-induced NFAT2 expression in T cells thereby limiting effector cytokine expression', Journal of Immunology, vol. 183, no. 2, pp. 907-915. https://doi.org/10.4049/jimmunol.0800216
Torgerson, Troy R. ; Genin, Anna ; Chen, Chunxia ; Zhang, Mingce ; Zhou, Bin ; Añover-Sombke, Stephanie ; Frank, M. Barton ; Dozmorov, Igor ; Ocheltree, Elizabeth ; Kulmala, Petri ; Centola, Michael ; Ochs, Hans D. ; Wells, Andrew D. ; Cron, Randy Q. / FOXP3 inhibits activation-induced NFAT2 expression in T cells thereby limiting effector cytokine expression. In: Journal of Immunology. 2009 ; Vol. 183, No. 2. pp. 907-915.
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