Four specific immunoglobulin domains in UNC-52/perlecan function with NID-1/nidogen during dendrite morphogenesis in caenorhabditis elegans

Kevin Celestrin, Carlos A. Díaz-Balzac, Leo T.H. Tang, Brian D. Ackley, Hannes E. Buelow

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The extracellular matrix is essential for various aspects of nervous system patterning. For example, sensory dendrites in flies, worms and fish have been shown to rely on coordinated interactions of tissues with extracellular matrix proteins. Here we show that the conserved basement membrane protein UNC-52/Perlecan is required for establishing the correct number of the highly ordered dendritic trees in the somatosensory neuron PVD in Caenorhabditis elegans. This function is dependent on four specific immunoglobulin domains, but independent of the known functions of UNC-52 in mediating muscleskin attachment. Intriguingly, the four conserved immunoglobulin domains in UNC-52 are necessary to correctly localize the basement membrane protein NID-1/Nidogen. Genetic experiments further show that unc-52, nid-1 and genes of the netrin axon guidance signaling cassette share a common pathway to establish the correct number of somatosensory dendrites. Our studies suggest that, in addition to its role in mediating muscle-skin attachment, UNC-52 functions through immunoglobulin domains to establish an ordered lattice of basement membrane proteins, which may control the function of morphogens during dendrite patterning.

Original languageEnglish (US)
Article numberdev158881
JournalDevelopment (Cambridge)
Volume145
Issue number10
DOIs
StatePublished - May 1 2018

Fingerprint

Caenorhabditis elegans
Dendrites
Morphogenesis
Basement Membrane
Membrane Proteins
Extracellular Matrix Proteins
Diptera
Nervous System
Extracellular Matrix
Fishes
Neurons
Muscles
Skin
Genes
perlecan
nidogen
Immunoglobulin Domains

Keywords

  • C. elegans
  • Dendrite
  • Nervous system development
  • Netrin
  • Nidogen
  • Perlecan

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Four specific immunoglobulin domains in UNC-52/perlecan function with NID-1/nidogen during dendrite morphogenesis in caenorhabditis elegans. / Celestrin, Kevin; Díaz-Balzac, Carlos A.; Tang, Leo T.H.; Ackley, Brian D.; Buelow, Hannes E.

In: Development (Cambridge), Vol. 145, No. 10, dev158881, 01.05.2018.

Research output: Contribution to journalArticle

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