Forward scattering particle image velocimetry (FSPIV): Application of Mie and imaging theory to measure 3D velocities in microscopic flows using partially coherent illumination and high aperture optics

Ben Ovryn, John D. Khaydarov

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We have combined Mie scattering theory and image theory to predict the forward scattering of light from spherical particles in a seeded fluid using high numerical aperture collection optics. Using this method, it is possible to determine all three components of a fluid's velocity by measuring the scattering from homogeneous spherical particles without moving the optics. The transverse velocity component is determined by following the centroid of the scattering pattern (with respect to time), while the component along the optical axis is determined by comparing the experimental data with numerical computations. We have verified our theoretical model and computer code by measuring the scattering from polystyrene particles illuminated with partially coherent, Koehler illumination in a transmitted light microscope. The three-dimensional scattering data is in quite good agreement with our model. To further verify our approach, we have measured the three- dimensional (parabolic) profile of a parallel flow of a low viscosity, seeded fluid in a straight channel (6 mm x 48 mm x 0.315 mm). The channel was placed on the stage of a conventional microscope equipped with a long working distance microscope objective, with the narrow dimension along the optical axis (OA). Instead of directly imaging the seed particles, the forward scattered light is recorded from the spherical, polystyrene seed particles (7 μm diameter).

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Pages243-254
Number of pages12
Volume2984
DOIs
StatePublished - 1997
Externally publishedYes
EventThree-Dimensional Microscopy: Image Acquisition and Processing IV - San Jose, CA, United States
Duration: Dec 2 1997Dec 2 1997

Other

OtherThree-Dimensional Microscopy: Image Acquisition and Processing IV
CountryUnited States
CitySan Jose, CA
Period12/2/9712/2/97

Fingerprint

Forward scattering
Mie scattering
forward scattering
particle image velocimetry
Velocity measurement
Optics
Illumination
Lighting
apertures
illumination
Imaging
Scattering
optics
Imaging techniques
Microscope
Mie Theory
Microscopes
Polystyrenes
microscopes
scattering

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Forward scattering particle image velocimetry (FSPIV) : Application of Mie and imaging theory to measure 3D velocities in microscopic flows using partially coherent illumination and high aperture optics. / Ovryn, Ben; Khaydarov, John D.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2984 1997. p. 243-254.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ovryn, B & Khaydarov, JD 1997, Forward scattering particle image velocimetry (FSPIV): Application of Mie and imaging theory to measure 3D velocities in microscopic flows using partially coherent illumination and high aperture optics. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 2984, pp. 243-254, Three-Dimensional Microscopy: Image Acquisition and Processing IV, San Jose, CA, United States, 12/2/97. https://doi.org/10.1117/12.271265
Ovryn, Ben ; Khaydarov, John D. / Forward scattering particle image velocimetry (FSPIV) : Application of Mie and imaging theory to measure 3D velocities in microscopic flows using partially coherent illumination and high aperture optics. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2984 1997. pp. 243-254
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