Following tracks of hemichannels

Rolf Dermietzel, Carola Meier, Feliksas Bukauskas, David C. Spray

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

It has been suggested that plasma membrane-bound hemichannels perform physiological and pathophysiological functions per se. Such functions require the presence of hemichannels on the cell surface and their accessibility to the extracellular environment for at least some limited period of time. We have previously shown that hemichannels can be labeled by means of antibodies directed to an external loop domain of connexin (Cx) 43 (1). We now provide evidence that trafficking of hemichannel vesicles can be visualized upon binding of a labeled homophilic peptide corresponding to a region of the first extracellular loop (EL1) of Cx43. In vivo imaging was performed after labeling hemichannels from the extracellular site with a mimetic peptide tagged with a fluorochrome (Alexa-546). Using a Cx43-CFP transfected HeLa cell line for incubation with the mimetic peptide, a significant number of double-labeled vesicles were found inside the cells. This double labeling indicates that a portion of Cx43 within the cell had accessed the cell surface as hemichannels where it bound to the peptide and was subsequently endocytosed. Pulse labeling with the peptide showed a decrease in the number of dual-labeled vesicles over time, indicating degradation and/or concurrent recycling of hemichannel vesicles.

Original languageEnglish (US)
Pages (from-to)335-340
Number of pages6
JournalCell Communication and Adhesion
Volume10
Issue number4-6
StatePublished - Jul 2003

Fingerprint

Connexin 43
Peptides
Labeling
Recycling
Cell membranes
Endocytosis
Fluorescent Dyes
HeLa Cells
Cells
Cell Membrane
Imaging techniques
Cell Line
Degradation
Antibodies

Keywords

  • Connexin hemichannels
  • Gap junction
  • Trafficking

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry

Cite this

Dermietzel, R., Meier, C., Bukauskas, F., & Spray, D. C. (2003). Following tracks of hemichannels. Cell Communication and Adhesion, 10(4-6), 335-340.

Following tracks of hemichannels. / Dermietzel, Rolf; Meier, Carola; Bukauskas, Feliksas; Spray, David C.

In: Cell Communication and Adhesion, Vol. 10, No. 4-6, 07.2003, p. 335-340.

Research output: Contribution to journalArticle

Dermietzel, R, Meier, C, Bukauskas, F & Spray, DC 2003, 'Following tracks of hemichannels', Cell Communication and Adhesion, vol. 10, no. 4-6, pp. 335-340.
Dermietzel R, Meier C, Bukauskas F, Spray DC. Following tracks of hemichannels. Cell Communication and Adhesion. 2003 Jul;10(4-6):335-340.
Dermietzel, Rolf ; Meier, Carola ; Bukauskas, Feliksas ; Spray, David C. / Following tracks of hemichannels. In: Cell Communication and Adhesion. 2003 ; Vol. 10, No. 4-6. pp. 335-340.
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