Following the folding of RNA with time-resolved synchrotron X-ray footprinting

Bianca Sclavi, Sarah Woodson, Michael Sullivan, Mark Chance, Michael D. Brenowitz

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The rapid mixing synchrotron X-ray footprinting technique described in this article allows nucleic acid folding and ligand binding reactions to be followed on a millisecond time resolution with single nucleotide resolution. In principle, the change in ·OH protection of every nucleotide in a nucleic acid hundreds of nucleotides long can be monitored separately. In addition, a wide range of solution conditions are compatible with the radiolytic generation of ·OH. These characteristics of synchrotron X-ray footprinting create opportunities for conducting thermodynamic and kinetic studies of nucleic acids that are both comprehensive and detailed. Kinetic footprinting studies of a number of systems have been initiated by the Center for Synchrotron Biosciences using this technique.

Original languageEnglish (US)
Pages (from-to)379-402
Number of pages24
JournalMethods in Enzymology
Volume295
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

RNA Folding
Synchrotrons
Nucleic Acids
Nucleotides
X-Rays
RNA
X rays
Kinetics
Thermodynamics
Ligands

ASJC Scopus subject areas

  • Biochemistry

Cite this

Following the folding of RNA with time-resolved synchrotron X-ray footprinting. / Sclavi, Bianca; Woodson, Sarah; Sullivan, Michael; Chance, Mark; Brenowitz, Michael D.

In: Methods in Enzymology, Vol. 295, 1998, p. 379-402.

Research output: Contribution to journalArticle

Sclavi, Bianca ; Woodson, Sarah ; Sullivan, Michael ; Chance, Mark ; Brenowitz, Michael D. / Following the folding of RNA with time-resolved synchrotron X-ray footprinting. In: Methods in Enzymology. 1998 ; Vol. 295. pp. 379-402.
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