Fluorescent proteins

Physical-chemical properties and application in cell biology

Olesya V. Stepanenko, Vladislav Verkhusha, I. M. Kuznetsova, K. K. Turoverov

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Green fluorescent protein (GFP) from jellyfish Aequorea victoria is the most extensively studied and widely used in cell biology protein. At present novel naturally occurring GFP-like proteins have been discovered and enhanced mutants of Aequorea GFP have been created. These mutants differ from wild-type GFP by stability, value of quantum yield, absorption and fluorescence spectra position and photochemical properties. GFP-like proteins are the fast growing family. This review is an attempt to characterize the main groups of GFP-like proteins, describe their structure and mechanisms of chromophore formation and summarize the main trends of their utilization as markers and biosensors in cell and molecular biology.

Original languageEnglish (US)
Pages (from-to)395-420
Number of pages26
JournalTsitologiya
Volume49
Issue number5
StatePublished - 2007

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Green Fluorescent Proteins
Cell Biology
Proteins
Protein Stability
Biosensing Techniques
Molecular Biology
Fluorescence

Keywords

  • Chromoproteins
  • Fluorescent proteins
  • Photoactivated proteins

ASJC Scopus subject areas

  • Histology

Cite this

Stepanenko, O. V., Verkhusha, V., Kuznetsova, I. M., & Turoverov, K. K. (2007). Fluorescent proteins: Physical-chemical properties and application in cell biology. Tsitologiya, 49(5), 395-420.

Fluorescent proteins : Physical-chemical properties and application in cell biology. / Stepanenko, Olesya V.; Verkhusha, Vladislav; Kuznetsova, I. M.; Turoverov, K. K.

In: Tsitologiya, Vol. 49, No. 5, 2007, p. 395-420.

Research output: Contribution to journalArticle

Stepanenko, OV, Verkhusha, V, Kuznetsova, IM & Turoverov, KK 2007, 'Fluorescent proteins: Physical-chemical properties and application in cell biology', Tsitologiya, vol. 49, no. 5, pp. 395-420.
Stepanenko, Olesya V. ; Verkhusha, Vladislav ; Kuznetsova, I. M. ; Turoverov, K. K. / Fluorescent proteins : Physical-chemical properties and application in cell biology. In: Tsitologiya. 2007 ; Vol. 49, No. 5. pp. 395-420.
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