Fluorescent proteins: Physical-chemical properties and application in cell biology

Olesya V. Stepanenko, V. V. Verkhusha, I. M. Kuznetsova, K. K. Turoverov

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Green fluorescent protein (GFP) from jellyfish Aequorea victoria is the most extensively studied and widely used in cell biology protein. At present novel naturally occurring GFP-like proteins have been discovered and enhanced mutants of Aequorea GFP have been created. These mutants differ from wild-type GFP by stability, value of quantum yield, absorption and fluorescence spectra position and photochemical properties. GFP-like proteins are the fast growing family. This review is an attempt to characterize the main groups of GFP-like proteins, describe their structure and mechanisms of chromophore formation and summarize the main trends of their utilization as markers and biosensors in cell and molecular biology.

Original languageEnglish (US)
Pages (from-to)395-420
Number of pages26
Issue number5
StatePublished - 2007


  • Chromoproteins
  • Fluorescent proteins
  • Photoactivated proteins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology


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