TY - JOUR
T1 - Fluorescent proteins
T2 - a cell biologist's user guide
AU - Snapp, Erik Lee
N1 - Funding Information:
Erik Snapp is an Ellison Medical Foundation New Scholar in Aging and is supported by NIA 1R21AG032544-01 and NIDDK 2PO1DK041918-16. Erik Snapp is a member of the Albert Einstein College of Medicine Marion Bessin Liver Center.
PY - 2009/11
Y1 - 2009/11
N2 - Fluorescent Proteins (FPs) have revolutionized cell biology. The value of labeling and visualizing proteins in living cells is evident from the thousands of publications since the cloning of Green Fluorescent Protein (GFP). Biologists have been flooded with a cornucopia of FPs; however, the FP toolbox has not necessarily been optimized for cell biologists. Common FP plasmids are suboptimal for the construction of proteins fused to FP. More problematic are commercial and investigator-constructed FP-fusion proteins that disrupt important cellular targeting information. Even when cell biologists correctly construct FP-fusion proteins, it is rarely self-evident which FP should be used. Important FP information, such as oligomer formation or photostability, is often obscure or anecdotal. This brief guide is offered to assist the biologist to exploit FPs in the analysis of cellular processes.
AB - Fluorescent Proteins (FPs) have revolutionized cell biology. The value of labeling and visualizing proteins in living cells is evident from the thousands of publications since the cloning of Green Fluorescent Protein (GFP). Biologists have been flooded with a cornucopia of FPs; however, the FP toolbox has not necessarily been optimized for cell biologists. Common FP plasmids are suboptimal for the construction of proteins fused to FP. More problematic are commercial and investigator-constructed FP-fusion proteins that disrupt important cellular targeting information. Even when cell biologists correctly construct FP-fusion proteins, it is rarely self-evident which FP should be used. Important FP information, such as oligomer formation or photostability, is often obscure or anecdotal. This brief guide is offered to assist the biologist to exploit FPs in the analysis of cellular processes.
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U2 - 10.1016/j.tcb.2009.08.002
DO - 10.1016/j.tcb.2009.08.002
M3 - Article
C2 - 19819147
AN - SCOPUS:70350337095
SN - 0962-8924
VL - 19
SP - 649
EP - 655
JO - Trends in Cell Biology
JF - Trends in Cell Biology
IS - 11
ER -