Fluorescent in situ hybridization assessment of chromosome 7 copy number in uncultured lung and kidney cells

Rizwan C. Naeem, Kevin Donovan, Joseph M. Corson, Jonathan A. Fletcher

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Trisomy 7 is common in cells cultured from nonneoplastic lung and kidney tissues, but the frequency of trisomy 7 in uncultured lung and kidney has not been determined. In this study, we used fluorescent in situ hybridization (FISH) to assess chromosome 7 copy number in uncultured interphase nuclei from lung and kidney specimens. All specimens had low level mosaicism for trisomy 7 (1.5-5.25%), but control experiments indicated a potential wide margin of error in quantifying these events.

Original languageEnglish (US)
Pages (from-to)100-102
Number of pages3
JournalCancer Genetics and Cytogenetics
Volume66
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

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Chromosomes, Human, Pair 7
Fluorescence In Situ Hybridization
Trisomy
Kidney
Lung
Interphase
Cultured Cells

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Fluorescent in situ hybridization assessment of chromosome 7 copy number in uncultured lung and kidney cells. / Naeem, Rizwan C.; Donovan, Kevin; Corson, Joseph M.; Fletcher, Jonathan A.

In: Cancer Genetics and Cytogenetics, Vol. 66, No. 2, 1993, p. 100-102.

Research output: Contribution to journalArticle

Naeem, Rizwan C. ; Donovan, Kevin ; Corson, Joseph M. ; Fletcher, Jonathan A. / Fluorescent in situ hybridization assessment of chromosome 7 copy number in uncultured lung and kidney cells. In: Cancer Genetics and Cytogenetics. 1993 ; Vol. 66, No. 2. pp. 100-102.
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