Fluorescence in situ hybridization with concomitant immunofluorescence in human pancreas

Jody Ye, Kathleen M. Gillespie

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The ability to identify the presence of non-host cells in human pancreas with concomitant characterization of cell phenotype is particularly important to facilitate studies of transplantation and microchimerism resulted from pregnancy. The steps involved in processing tissue for fluorescence in situ hybridization (FISH) can however remove epitopes that are crucial for immunofluorescence and antigen retrieval strategies for immunofluorescence can negatively influence FISH. We describe a robust method to analyze X/Y chromosome constitution and cell phenotype simultaneously on the same pancreatic tissue section.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages153-158
Number of pages6
DOIs
StatePublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1433
ISSN (Print)1064-3745

Keywords

  • Fluorescence in situ hybridization
  • Microchimerism
  • Transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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