Fluctuations in self-assembled sickle hemoglobin fibers

Matthew S. Turner, Jiangcheng Wang, Christopher W. Jones, Frank A. Ferrone, Robert Josephs, Robin W. Briehl

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We discuss microscopic measurements of thermal fluctuations in self-assembled sickle hemoglobin fibers. These permit the measurement of various mechanical moduli that control the rheology of the fiber gel responsible for the pathology of the disease. Differential interference contrast microscopy of isolated fibers undergoing equilibrium thermal bending fluctuations leads to estimates of thermal persistence lengths of between 0.23 and 10.4 mm for the most flexible and stiffest fibers, respectively. We argue that this large range may reflect the formation of fiber bundles. If the most flexible fibers are single fibers, then the stiffest measured object is consistent with a "closed shell" hexagonal bundle of 7 single fibers. We estimate the spectrum of persistence lengths associated with bundles consisting of different numbers of single fibers and compare this with the experimental data. The equivalent Young'S modulus for the material is 0.1 GPa, less than for structural proteins but much larger than for extensible proteins.

Original languageEnglish (US)
Pages (from-to)7182-7187
Number of pages6
JournalLangmuir
Volume18
Issue number19
DOIs
StatePublished - Sep 17 2002

Fingerprint

Sickle Hemoglobin
Hemoglobin
hemoglobin
fibers
Fibers
bundles
proteins
Proteins
pathology
Pathology
estimates
Rheology
rheology
Microscopic examination
Gels
gels
microscopy
interference

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

Turner, M. S., Wang, J., Jones, C. W., Ferrone, F. A., Josephs, R., & Briehl, R. W. (2002). Fluctuations in self-assembled sickle hemoglobin fibers. Langmuir, 18(19), 7182-7187. https://doi.org/10.1021/la025539k

Fluctuations in self-assembled sickle hemoglobin fibers. / Turner, Matthew S.; Wang, Jiangcheng; Jones, Christopher W.; Ferrone, Frank A.; Josephs, Robert; Briehl, Robin W.

In: Langmuir, Vol. 18, No. 19, 17.09.2002, p. 7182-7187.

Research output: Contribution to journalArticle

Turner, MS, Wang, J, Jones, CW, Ferrone, FA, Josephs, R & Briehl, RW 2002, 'Fluctuations in self-assembled sickle hemoglobin fibers', Langmuir, vol. 18, no. 19, pp. 7182-7187. https://doi.org/10.1021/la025539k
Turner MS, Wang J, Jones CW, Ferrone FA, Josephs R, Briehl RW. Fluctuations in self-assembled sickle hemoglobin fibers. Langmuir. 2002 Sep 17;18(19):7182-7187. https://doi.org/10.1021/la025539k
Turner, Matthew S. ; Wang, Jiangcheng ; Jones, Christopher W. ; Ferrone, Frank A. ; Josephs, Robert ; Briehl, Robin W. / Fluctuations in self-assembled sickle hemoglobin fibers. In: Langmuir. 2002 ; Vol. 18, No. 19. pp. 7182-7187.
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