Flow inconsistency

The evil twin of column switching-Hardware aspects

Eduard Rogatsky, Kara Braaten, Greg Cruikshank, Harsha Jayatillake, BingNa Zheng, Daniel T. Stein

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Solvent flow, generated by HPLC pumps is consistent and accurate. This statement, while true for single column (one dimensional) liquid chromatography applications, may not apply to column switching applications. Connection of pumps and/or columns to one flow path may cause substantial pressure changes. Immediate post valve switch pressure differences between pumps can cause backflow where the mobile phase stored at higher pressure will temporary flow into the lower pressure area. A more common side effect of column switching is flow inconsistency during pump pressurization. For the duration of pump pressurization, liquid flow through the column will be smaller than expected since the HPLC column acts like a flow restrictor.

Original languageEnglish (US)
Pages (from-to)7721-7727
Number of pages7
JournalJournal of Chromatography A
Volume1216
Issue number45
DOIs
StatePublished - Nov 6 2009

Fingerprint

Pumps
Hardware
Pressure
Pressurization
High Pressure Liquid Chromatography
Liquid Chromatography
Liquid chromatography
Switches
Liquids

Keywords

  • Column switching
  • Dead (delay) volume
  • Flow inconsistency
  • Focus mode
  • Pressurization
  • Turbulent flow chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

Flow inconsistency : The evil twin of column switching-Hardware aspects. / Rogatsky, Eduard; Braaten, Kara; Cruikshank, Greg; Jayatillake, Harsha; Zheng, BingNa; Stein, Daniel T.

In: Journal of Chromatography A, Vol. 1216, No. 45, 06.11.2009, p. 7721-7727.

Research output: Contribution to journalArticle

Rogatsky, E, Braaten, K, Cruikshank, G, Jayatillake, H, Zheng, B & Stein, DT 2009, 'Flow inconsistency: The evil twin of column switching-Hardware aspects', Journal of Chromatography A, vol. 1216, no. 45, pp. 7721-7727. https://doi.org/10.1016/j.chroma.2009.08.078
Rogatsky, Eduard ; Braaten, Kara ; Cruikshank, Greg ; Jayatillake, Harsha ; Zheng, BingNa ; Stein, Daniel T. / Flow inconsistency : The evil twin of column switching-Hardware aspects. In: Journal of Chromatography A. 2009 ; Vol. 1216, No. 45. pp. 7721-7727.
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