Flow cytometry of fluorescent proteins

William G. Telford; Teresa Hawley; Fedor Subach; Vladislav Verkhusha; Robert G. Hawley

doi:10.1016/j.ymeth.2012.01.003

Flow cytometry of fluorescent proteins

William G. Telford, Teresa Hawley, Fedor Subach, Vladislav Verkhusha, Robert G. Hawley

Genetics

Research output: Contribution to journal › Review article › peer-review

71 Scopus citations

Abstract

Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.

Original language	English (US)
Pages (from-to)	318-330
Number of pages	13
Journal	Methods
Volume	57
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2012.01.003
State	Published - Jul 2012

Keywords

CFP
DsRed
Flow cytometry
Fluorescent protein
GFP
MCherry
YFP

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.ymeth.2012.01.003

Cite this

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title = "Flow cytometry of fluorescent proteins",

abstract = "Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.",

keywords = "CFP, DsRed, Flow cytometry, Fluorescent protein, GFP, MCherry, YFP",

author = "Telford, {William G.} and Teresa Hawley and Fedor Subach and Vladislav Verkhusha and Hawley, {Robert G.}",

year = "2012",

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AU - Telford, William G.

AU - Hawley, Teresa

AU - Subach, Fedor

AU - Verkhusha, Vladislav

AU - Hawley, Robert G.

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AB - Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.

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KW - DsRed

KW - Flow cytometry

KW - Fluorescent protein

KW - GFP

KW - MCherry

KW - YFP

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JO - Methods

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Flow cytometry of fluorescent proteins

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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