Flow cytometry of fluorescent proteins

William G. Telford, Teresa Hawley, Fedor Subach, Vladislav Verkhusha, Robert G. Hawley

Research output: Contribution to journalReview article

44 Scopus citations

Abstract

Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.

Original languageEnglish (US)
Pages (from-to)318-330
Number of pages13
JournalMethods
Volume57
Issue number3
DOIs
StatePublished - Jul 1 2012

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Keywords

  • CFP
  • DsRed
  • Flow cytometry
  • Fluorescent protein
  • GFP
  • MCherry
  • YFP

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Telford, W. G., Hawley, T., Subach, F., Verkhusha, V., & Hawley, R. G. (2012). Flow cytometry of fluorescent proteins. Methods, 57(3), 318-330. https://doi.org/10.1016/j.ymeth.2012.01.003