Flow cytometry in hairy cell leukemia before and during interferon alfa-2b therapy

E. E. Vokes, M. A. Bitter, M. J. Ratain, Michael B. Prystowsky, K. Daly, H. M. Golomb

Research output: Contribution to journalArticle

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Abstract

Mononuclear cells from 15 patients with hairy cell leukemia were studied before and during therapy with interferon alfa-2b (IFN) by regular peripheral blood differential counts and flow cytometry, using a panel of monoclonal antibodies (Moab). Seven leukemic phase patients (Group 1) had a mean leukocyte count of 48,870/μl at entry with a mean absolute hairy cell (HC) count of 40,100/μl. After 3 months on IFN, both parameters decreased significantly (WBC 3,500/μl; HC count 130/μl). In eight patients with a cytopenic form of the disease (Group 2) the mean leukocyte count rose from 2950/μl to 3890/μl while the mean absolute HC count decreased from 300/μl to 120/μl. The morphologic shifts correlated well with changes in the Moab reaction pattern. In Group 1 the activity of all Moab decreased significantly. In Group 2, only cells expressing Leu 3a and Leu 11a (a marker of natural killer cells) showed a significant shift, the latter increasing from 170/μl to 360/μl. This increase in natural killer cell antigen expression was not obvious based on routine morphologic observations alone. We show that flow cytometry may be a useful adjunct in monitoring the response of HCL to therapy. Changes in populations of cells that may be difficult to discriminate on morphologic grounds alone may be observed.

Original languageEnglish (US)
Pages (from-to)1987-1991
Number of pages5
JournalCancer
Volume59
Issue number12
DOIs
StatePublished - 1987
Externally publishedYes

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interferon alfa-2b
Hairy Cell Leukemia
Flow Cytometry
Cell Count
Monoclonal Antibodies
Leukocyte Count
Natural Killer Cells
Therapeutics
Antigens
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Flow cytometry in hairy cell leukemia before and during interferon alfa-2b therapy. / Vokes, E. E.; Bitter, M. A.; Ratain, M. J.; Prystowsky, Michael B.; Daly, K.; Golomb, H. M.

In: Cancer, Vol. 59, No. 12, 1987, p. 1987-1991.

Research output: Contribution to journalArticle

Vokes, E. E. ; Bitter, M. A. ; Ratain, M. J. ; Prystowsky, Michael B. ; Daly, K. ; Golomb, H. M. / Flow cytometry in hairy cell leukemia before and during interferon alfa-2b therapy. In: Cancer. 1987 ; Vol. 59, No. 12. pp. 1987-1991.
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