Mononuclear cells from 15 patients with hairy cell leukemia were studied before and during therapy with interferon alfa‐2b (IFN) by regular peripheral blood differential counts and flow cytometry, using a panel of monoclonal antibodies (Moab). Seven leukemic phase patients (Group 1) had a mean leukocyte count of 48,870/μl at entry with a mean absolute hairy cell (HC) count of 40,100/μl. After 3 months on IFN, both parameters decreased significantly (WBC 3,500/μl; HC count 130/μl). In eight patients with a cytopenic form of the disease (Group 2) the mean leukocyte count rose from 2950/μl to 3890/μl while the mean absolute HC count decreased from 300/μl to 120/μl. The morphologic shifts correlated well with changes in the Moab reaction pattern. In Group 1 the activity of all Moab decreased significantly. In Group 2, only cells expressing Leu 3a and Leu 11a (a marker of natural killer cells) showed a significant shift, the latter increasing from 170/μl to 360/μl. This increase in natural killer cell antigen expression was not obvious based on routine morphologic observations alone. We show that flow cytometry may be a useful adjunct in monitoring the response of HCL to therapy. Changes in populations of cells that may be difficult to discriminate on morphologic grounds alone may be observed.
|Original language||English (US)|
|Number of pages||5|
|State||Published - Jun 15 1987|
ASJC Scopus subject areas
- Cancer Research
Flow cytometry in hairy cell leukemia before and during interferon alfa‐2b therapy. / Vokes, Everett E.; Bitter, Mitchell A.; Ratain, Mark J.; Prystowsky, Michael B.; Daly, Karen; Golomb, Harvey M.In: Cancer, Vol. 59, No. 12, 15.06.1987, p. 1987-1991.
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