Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation

V. Looser, F. Hammes, M. Keller, Michael Berney, K. Kovar, Thomas Egli

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The key to optimizing productivity during industrial fermentations is the ability to rapidly monitor and interpret the physiological state of single microbial cells in a population and to recognize and characterize different sub-populations. Here, a flow cytometry-based method for the reproducible detection of changes in membrane function and/or structure of recombinant E. coli JM101 (pSPZ3) expressing xylene monooxygenase (XMO), was developed. XMO expression led to compromised but not permeabilized cell membranes. This was deduced from the fact that recombinant cells only stained with ethidium bromide (EB) and not with propidium iodide (PI). During the glucose-limited fedbatch cultivation, an increase from 25% to 95% of EB-stained cells was observed, occurring between 2 and 5 h after induction. Control experiments confirmed that this increase was due to the recombinant protein production and not caused by any possible effects of varying substrate availability, high cell density, plasmid replication or the presence of the inducing agent. We hypothesize that the integration of the recombinant protein into the cell membrane physically disrupted the functionality of the efflux pumps, thus resulting in EB-staining of the recombinant cells. This method enabled us to detect changes in the physiological state of single cells 2-4 h before other indications of partial cell damage, such as unbalanced growth, acetate accumulation and an increased CO2 production rate, were observed. This method therefore shows promise with respect to the further development of an early-warning system to prevent sudden productivity decreases in processes with recombinant E. coli expressing heterologous membrane proteins.

Original languageEnglish (US)
Pages (from-to)69-78
Number of pages10
JournalBiotechnology and Bioengineering
Volume92
Issue number1
DOIs
StatePublished - Oct 5 2005
Externally publishedYes

Fingerprint

Recombinant proteins
Ethidium
Cell membranes
Xylene
Escherichia coli
Membrane Proteins
Carbon
Productivity
Proteins
Membranes
Recombinant Proteins
Flow cytometry
Alarm systems
Fermentation
Glucose
Propidium
Cells
Availability
Pumps
Acetates

Keywords

  • E. coli
  • Ethidium bromide
  • Fedbatch cultivation
  • Flow cytometry
  • Physiological state
  • Propidium iodide
  • Recombinant membrane protein
  • Xylene monooxygenase

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation. / Looser, V.; Hammes, F.; Keller, M.; Berney, Michael; Kovar, K.; Egli, Thomas.

In: Biotechnology and Bioengineering, Vol. 92, No. 1, 05.10.2005, p. 69-78.

Research output: Contribution to journalArticle

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