Flow cytometric analysis of the DNA distribution in human brain tumors

K. Kawamoto, F. Herz, R. C. Wolley, A. Hirano, H. Kajikawa, L. G. Koss

Research output: Contribution to journalArticle

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Abstract

Flow cytofluorometric analysis was used to determine the distribution of the DNA content in cells from selected areas of normal human brain and in benign and malignant brain tumors. Propidium iodide was employed as DNA fluorochrome and the analysis was carried out on a suspension of single cells. Normal, nonstimulated human lymphocytes were used as diploid controls. With nonneoplastic tissue an average of 91% of the cells were diploid (presumably in G0 or G1 stage of the cell cycle). The cells of most benign tumors were mainly diploid (77-98%), nine specimens of pituitary adenomas had large numbers of aneuploid cells. In glioblastoma multiforme the proportion of diploid cells was significantly diminished and polyploid cells were frequently seen. Similar results were obtained in other malignant tumors, with metastatic tumors showing the greatest ploidy variation, which included triploid, tetraploid, and hypertetraploid cells. The analytical method used provides valuable information of significant clinical importance on the DNA distribution in brain tumor cells.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalActa Neuropathologica
Volume46
Issue number1-2
DOIs
StatePublished - Jan 1979

Fingerprint

Brain Neoplasms
Diploidy
DNA
Triploidy
Neoplasms
Polyploidy
Tetraploidy
Propidium
Ploidies
Pituitary Neoplasms
Aneuploidy
Glioblastoma
Fluorescent Dyes
Suspensions
Cell Cycle
Lymphocytes
Brain

Keywords

  • Brain tumor
  • DNA distribution
  • Flow cytometry

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pathology and Forensic Medicine
  • Clinical Neurology

Cite this

Kawamoto, K., Herz, F., Wolley, R. C., Hirano, A., Kajikawa, H., & Koss, L. G. (1979). Flow cytometric analysis of the DNA distribution in human brain tumors. Acta Neuropathologica, 46(1-2), 39-44. https://doi.org/10.1007/BF00684802

Flow cytometric analysis of the DNA distribution in human brain tumors. / Kawamoto, K.; Herz, F.; Wolley, R. C.; Hirano, A.; Kajikawa, H.; Koss, L. G.

In: Acta Neuropathologica, Vol. 46, No. 1-2, 01.1979, p. 39-44.

Research output: Contribution to journalArticle

Kawamoto, K, Herz, F, Wolley, RC, Hirano, A, Kajikawa, H & Koss, LG 1979, 'Flow cytometric analysis of the DNA distribution in human brain tumors', Acta Neuropathologica, vol. 46, no. 1-2, pp. 39-44. https://doi.org/10.1007/BF00684802
Kawamoto K, Herz F, Wolley RC, Hirano A, Kajikawa H, Koss LG. Flow cytometric analysis of the DNA distribution in human brain tumors. Acta Neuropathologica. 1979 Jan;46(1-2):39-44. https://doi.org/10.1007/BF00684802
Kawamoto, K. ; Herz, F. ; Wolley, R. C. ; Hirano, A. ; Kajikawa, H. ; Koss, L. G. / Flow cytometric analysis of the DNA distribution in human brain tumors. In: Acta Neuropathologica. 1979 ; Vol. 46, No. 1-2. pp. 39-44.
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