Abstract
Analysis of DNA content in cultured cells derived from 5 benign and 8 malignant human brain tumors was performed by flow cytometry, using propidium iodide as fluorochrome. Normal, non-stimulated human lymphocytes were used as controls. Cells harvested from the first confluent subculture had DNA distribution histograms similar to those of a replicating, non-synchronous population of diploid cells. This observation was made on all cultures, regardless of the DNA distribution patterns in the original tumors, including two metastatic tumors which originally had a predominantly triploid DNA content. After 3 to 5 transfers an increase in the proportion of cells with a wide range of DNA content was observed in some cultures. These changes were usually associated with a loss of cell viability. These observations suggest that in the experimental setting described, in vitro proliferation of human tumor cells may be selectively associated with cells with diploid amounts of DNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11-17 |
| Number of pages | 7 |
| Journal | Virchows Archiv B Cell Pathology Including Molecular Pathology |
| Volume | 35 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 1980 |
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Keywords
- Brain tumors
- Cultured cells
- DNA
- Flow cytometry
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Medicine(all)
Cite this
Flow cytometric analysis of the DNA content in cultured human brain tumor cells. / Kawamoto, Keiji; Herz, Fritz; Wolley, Robert C.; Hirano, Asao; Koss, Leopold G.
In: Virchows Archiv B Cell Pathology Including Molecular Pathology, Vol. 35, No. 1, 12.1980, p. 11-17.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Flow cytometric analysis of the DNA content in cultured human brain tumor cells
AU - Kawamoto, Keiji
AU - Herz, Fritz
AU - Wolley, Robert C.
AU - Hirano, Asao
AU - Koss, Leopold G.
PY - 1980/12
Y1 - 1980/12
N2 - Analysis of DNA content in cultured cells derived from 5 benign and 8 malignant human brain tumors was performed by flow cytometry, using propidium iodide as fluorochrome. Normal, non-stimulated human lymphocytes were used as controls. Cells harvested from the first confluent subculture had DNA distribution histograms similar to those of a replicating, non-synchronous population of diploid cells. This observation was made on all cultures, regardless of the DNA distribution patterns in the original tumors, including two metastatic tumors which originally had a predominantly triploid DNA content. After 3 to 5 transfers an increase in the proportion of cells with a wide range of DNA content was observed in some cultures. These changes were usually associated with a loss of cell viability. These observations suggest that in the experimental setting described, in vitro proliferation of human tumor cells may be selectively associated with cells with diploid amounts of DNA.
AB - Analysis of DNA content in cultured cells derived from 5 benign and 8 malignant human brain tumors was performed by flow cytometry, using propidium iodide as fluorochrome. Normal, non-stimulated human lymphocytes were used as controls. Cells harvested from the first confluent subculture had DNA distribution histograms similar to those of a replicating, non-synchronous population of diploid cells. This observation was made on all cultures, regardless of the DNA distribution patterns in the original tumors, including two metastatic tumors which originally had a predominantly triploid DNA content. After 3 to 5 transfers an increase in the proportion of cells with a wide range of DNA content was observed in some cultures. These changes were usually associated with a loss of cell viability. These observations suggest that in the experimental setting described, in vitro proliferation of human tumor cells may be selectively associated with cells with diploid amounts of DNA.
KW - Brain tumors
KW - Cultured cells
KW - DNA
KW - Flow cytometry
UR - http://www.scopus.com/inward/record.url?scp=0019157308&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019157308&partnerID=8YFLogxK
U2 - 10.1007/BF02889145
DO - 10.1007/BF02889145
M3 - Article
C2 - 6111153
AN - SCOPUS:0019157308
VL - 35
SP - 11
EP - 17
JO - Virchows Archiv B Cell Pathology Including Molecular Pathology
JF - Virchows Archiv B Cell Pathology Including Molecular Pathology
SN - 0340-6075
IS - 1
ER -