Flavokawain B, a kava chalcone, induces apoptosis in synovial sarcoma cell lines

Toshinori Sakai, Ramez N. Eskander, Yi Guo, Kap Jung Kim, Jason Mefford, Justin Hopkins, Nitin N. Bhatia, Xiaolin Zi, Bang H. Hoang

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Synovial sarcomas (SS) are soft tissue sarcomas with poor prognosis, displaying a lack of response to conventional cytotoxic chemotherapy. Although SS cell lines have moderate chemosensitivity to isofamide and doxorubicin therapy, the clinical prognosis is still poor. In this article, we showed that flavokawain B (FKB), a novel chalcone from kava extract, potently inhibits the growth of SS cell lines SYO-I and HS-SY-II through induction of apoptosis. Treatment with FKB increased caspase 8, 9, and 3/7 activity compared to vehicle-treated controls, indicating that both extrinsic and intrinsic apoptotic pathways were activated. Furthermore, FKB treatment of both cell lines resulted in increased mRNA and protein expression of death receptor-5 and the mitochondrial pro-apoptotic proteins Bim and Puma, while down-regulating the expression of an inhibitor of apoptosis, survivin in a dose-dependent manner. Our results suggest the natural compound FKB has a pro-apoptotic effect on SS cell lines. FKB may be a new chemotherapeutic strategy for patients with SS and deserves further investigation as a potential agent in the treatment of this malignancy.

Original languageEnglish (US)
Pages (from-to)1045-1050
Number of pages6
JournalJournal of Orthopaedic Research
Volume30
Issue number7
DOIs
StatePublished - Jul 2012
Externally publishedYes

Fingerprint

Kava
Chalcone
Synovial Sarcoma
Apoptosis
Cell Line
Puma
TNF-Related Apoptosis-Inducing Ligand Receptors
Apoptosis Regulatory Proteins
Caspase 9
Caspase 8
Mitochondrial Proteins
Therapeutics
Caspase 3
Sarcoma
Doxorubicin
flavokawain B
Drug Therapy
Messenger RNA
Growth
Neoplasms

Keywords

  • apoptosis
  • flavokawain
  • synovial sarcoma

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Flavokawain B, a kava chalcone, induces apoptosis in synovial sarcoma cell lines. / Sakai, Toshinori; Eskander, Ramez N.; Guo, Yi; Kim, Kap Jung; Mefford, Jason; Hopkins, Justin; Bhatia, Nitin N.; Zi, Xiaolin; Hoang, Bang H.

In: Journal of Orthopaedic Research, Vol. 30, No. 7, 07.2012, p. 1045-1050.

Research output: Contribution to journalArticle

Sakai, T, Eskander, RN, Guo, Y, Kim, KJ, Mefford, J, Hopkins, J, Bhatia, NN, Zi, X & Hoang, BH 2012, 'Flavokawain B, a kava chalcone, induces apoptosis in synovial sarcoma cell lines', Journal of Orthopaedic Research, vol. 30, no. 7, pp. 1045-1050. https://doi.org/10.1002/jor.22050
Sakai, Toshinori ; Eskander, Ramez N. ; Guo, Yi ; Kim, Kap Jung ; Mefford, Jason ; Hopkins, Justin ; Bhatia, Nitin N. ; Zi, Xiaolin ; Hoang, Bang H. / Flavokawain B, a kava chalcone, induces apoptosis in synovial sarcoma cell lines. In: Journal of Orthopaedic Research. 2012 ; Vol. 30, No. 7. pp. 1045-1050.
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