Flavohemoglobin, a Globin with a Peroxidase-like Catalytic Site

Masahiro Mukai, Catherine E. Mills, Robert K. Poole, Syun-Ru Yeh

Research output: Contribution to journalArticle

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Abstract

Biochemical studies of flavohemoglobin (Hmp) from Escherichia coli suggest that instead of aerobic oxygen delivery, a dioxygenase converts NO to NO 3- and anaerobically, an NO reductase converts NO to N2O. To investigate the structural features underlying the chemical reactivity of Hmp, we have measured the resonance Raman spectra of the ligand-free ferric and ferrous protein and the CO derivatives of the ferrous protein. At neutral pH, the ferric protein has a five-coordinate high-spin heme, similar to peroxidases. In the ferrous protein, a strong iron-histidine stretching mode is present at 244 cm-1. This frequency is much higher than that of any other globin discovered to date, although it is comparable to those of peroxidases, suggesting that the proximal histidine has imidazolate character. In the CO derivative, an open and a closed conformation were detected. The distal environment of the closed conformation is very polar, where the heme-bound CO strongly interacts with the B10 Tyr and/or the E7 Gln. These data demonstrate that the active site structure of Hmp is very similar to that of peroxidases and is tailored to perform oxygen chemistry.

Original languageEnglish (US)
Pages (from-to)7272-7277
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number10
DOIs
StatePublished - Mar 9 2001

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Globins
Peroxidases
Peroxidase
Catalytic Domain
Carbon Monoxide
Heme
Histidine
Conformations
Proteins
Oxygen
Derivatives
Chemical reactivity
Escherichia coli
Stretching
Raman scattering
Oxidoreductases
Iron
Ligands

ASJC Scopus subject areas

  • Biochemistry

Cite this

Flavohemoglobin, a Globin with a Peroxidase-like Catalytic Site. / Mukai, Masahiro; Mills, Catherine E.; Poole, Robert K.; Yeh, Syun-Ru.

In: Journal of Biological Chemistry, Vol. 276, No. 10, 09.03.2001, p. 7272-7277.

Research output: Contribution to journalArticle

Mukai, Masahiro ; Mills, Catherine E. ; Poole, Robert K. ; Yeh, Syun-Ru. / Flavohemoglobin, a Globin with a Peroxidase-like Catalytic Site. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 10. pp. 7272-7277.
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