Flash photolysis of caged inositol 1,4,5-trisphosphate activates plasma membrane calcium current in human T cells

Thomas V. McDonald, Brett A. Premack, Phyllis Gardner

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Sustained elevation of intracellular Ca2+ by cell surface receptors is often dependent on influx of Ca2+ across the plasma membrane through routes not in-volving voltage-gated Ca2+ channels. We demonstrate that intracellular release of inositol 1,4,5-trisphosphate (InsP3), either from stimulation of transfected human muscarinic receptors or from photolytic release of caged InSP3, activates whole cell Ca2+ current in the Jurkat T cell line. Whole cell voltage clamp recordings indicate that the current is carried by a Ca2+-selective channel that resembles T-type voltage-gated Ca2+ channels in relative conductance of different cation species. Elevation of internal Ca2+ inactivates the channel, whereas internal perfusion with inositol 1,3,4,5-tetrakisphosphate (InsP4) does not affect t. Photolytic release of caged 1-(α-glycerophosphoryl)-inositol 4,5-bisphosphate, an analog of InsP3 which activates InsP3 receptors but is not readily metabolized to InsP4, also activates the current. We conclude that generation of InsP3 is sufficient to activate Ca2+-selective channels in the plasma membrane of T cells. InsP3 may have its effect indirectly through depletion of Ca2+ stores, or directly with a plasma membrane-associated InsP3 receptor.

Original languageEnglish (US)
Pages (from-to)3889-3896
Number of pages8
JournalJournal of Biological Chemistry
Volume268
Issue number6
StatePublished - Feb 25 1993
Externally publishedYes

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Inositol 1,4,5-Trisphosphate
T-cells
Photolysis
Cell membranes
Cell Membrane
Calcium
T-Lymphocytes
Electric potential
Jurkat Cells
Clamping devices
Cell Surface Receptors
Muscarinic Receptors
Cations
Perfusion
Cell Line

ASJC Scopus subject areas

  • Biochemistry

Cite this

Flash photolysis of caged inositol 1,4,5-trisphosphate activates plasma membrane calcium current in human T cells. / McDonald, Thomas V.; Premack, Brett A.; Gardner, Phyllis.

In: Journal of Biological Chemistry, Vol. 268, No. 6, 25.02.1993, p. 3889-3896.

Research output: Contribution to journalArticle

McDonald, Thomas V. ; Premack, Brett A. ; Gardner, Phyllis. / Flash photolysis of caged inositol 1,4,5-trisphosphate activates plasma membrane calcium current in human T cells. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 6. pp. 3889-3896.
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