Flanking DNA-sequences contribute to the specific binding of cl-repressor and ORI

Michael D. Brenowitz, Donald F. Senear, Gary K. Ackers

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The binding of cl-repressor to a series of mutant operators containing OR1 of the right operator of bacteriophage lambda was investigated. Sites OR2 and/or OR3 were inactivated by either point or deletion mutations. The free energy of binding repressor to OR1 in the wildtype operator, △G1, is -13.7 ± 0.3 kcal/mol. △G1, determined for an OR2- operator created by a single point mutation in OR2 is -13.6 ± 0.2 kcal/mol. In contrast, △G1, for the binding of repressor to a cloned synthetic OR1 operator containing only 24 bp of lambda sequence is -12.2 ± 0.1 kcal/mol. When sequence 5' to OR1 is present, the binding affinity increases to -13.0 ± 0.1 kcal/mol. In addition, the proximity of OR1 to a fragment-end decreases △G1, from -13.7 to -12.3 ± 0.1 kcal/mol. These results suggest that the DNA sequence outside the 17 bp OR1 binding-site contributes to the specific binding of cl-repressor.

Original languageEnglish (US)
Pages (from-to)3747-3755
Number of pages9
JournalNucleic Acids Research
Volume17
Issue number10
DOIs
StatePublished - May 25 1989
Externally publishedYes

Fingerprint

Bacteriophages
DNA sequences
Binding sites
Point Mutation
DNA Sequence
Free energy
Binding Sites
Bacteriophage lambda
Sequence Deletion
Operator
Mutation
Mutant
Deletion
Proximity
Affine transformation
Free Energy
Fragment
Decrease
Series

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Flanking DNA-sequences contribute to the specific binding of cl-repressor and ORI. / Brenowitz, Michael D.; Senear, Donald F.; Ackers, Gary K.

In: Nucleic Acids Research, Vol. 17, No. 10, 25.05.1989, p. 3747-3755.

Research output: Contribution to journalArticle

Brenowitz, Michael D. ; Senear, Donald F. ; Ackers, Gary K. / Flanking DNA-sequences contribute to the specific binding of cl-repressor and ORI. In: Nucleic Acids Research. 1989 ; Vol. 17, No. 10. pp. 3747-3755.
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