Fission yeast Int6 is not essential for global translation initiation, but deletion of int6+ causes hypersensitivity to caffeine and affects spore formation

A. Bandyopadhyay, T. Matsumoto, U. Maitra

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Mammalian INT6 protein has been considered to be a subunit of the eukaryotic translation initiation factor, eIF3. The Int6 locus is also known as a common integration site of mouse mammary tumor virus (MMTV). However, the function of Int6 in translation initiation and the mechanism of Int6-mediated tumor induction are yet to be explored. In this study, the fission yeast, Schizosaccharomyces pombe, int6+, which is 43% identical to the mammalian counterpart, was deleted. Despite the evidence that the majority of Int6 protein was associated with 40S particles in this organism, strains lacking int6+ (Δint6) were viable and showed only moderate inhibition in the rate of in vivo global protein synthesis. Polysome profile analysis showed no apparent defects in translation initiation. Δint6 exhibited a hypersensitivity to caffeine, which could be suppressed by the addition of sorbitol to the growth medium. This and other phenotypes would imply that int6+ is required for the integrity of cell membrane. In meiosis, Δint6 produced incomplete tetrads frequently. High dosage expression of a truncated mutant of int6+ conferred a hypersensitivity to caffeine, but did not cause the defect in meiosis. A possible link between the function of int6+ and the Δint6-phenotypes is discussed.

Original languageEnglish (US)
Pages (from-to)4005-4018
Number of pages14
JournalMolecular Biology of the Cell
Volume11
Issue number11
StatePublished - 2000

Fingerprint

Schizosaccharomyces
Meiosis
Caffeine
Spores
Eukaryotic Initiation Factor-3
Hypersensitivity
Eukaryotic Initiation Factors
Mouse mammary tumor virus
Phenotype
Polyribosomes
Sorbitol
Proteins
Cell Membrane
Growth
Neoplasms

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Fission yeast Int6 is not essential for global translation initiation, but deletion of int6+ causes hypersensitivity to caffeine and affects spore formation. / Bandyopadhyay, A.; Matsumoto, T.; Maitra, U.

In: Molecular Biology of the Cell, Vol. 11, No. 11, 2000, p. 4005-4018.

Research output: Contribution to journalArticle

@article{b45076a966a8445ead061121de81e2d3,
title = "Fission yeast Int6 is not essential for global translation initiation, but deletion of int6+ causes hypersensitivity to caffeine and affects spore formation",
abstract = "Mammalian INT6 protein has been considered to be a subunit of the eukaryotic translation initiation factor, eIF3. The Int6 locus is also known as a common integration site of mouse mammary tumor virus (MMTV). However, the function of Int6 in translation initiation and the mechanism of Int6-mediated tumor induction are yet to be explored. In this study, the fission yeast, Schizosaccharomyces pombe, int6+, which is 43{\%} identical to the mammalian counterpart, was deleted. Despite the evidence that the majority of Int6 protein was associated with 40S particles in this organism, strains lacking int6+ (Δint6) were viable and showed only moderate inhibition in the rate of in vivo global protein synthesis. Polysome profile analysis showed no apparent defects in translation initiation. Δint6 exhibited a hypersensitivity to caffeine, which could be suppressed by the addition of sorbitol to the growth medium. This and other phenotypes would imply that int6+ is required for the integrity of cell membrane. In meiosis, Δint6 produced incomplete tetrads frequently. High dosage expression of a truncated mutant of int6+ conferred a hypersensitivity to caffeine, but did not cause the defect in meiosis. A possible link between the function of int6+ and the Δint6-phenotypes is discussed.",
author = "A. Bandyopadhyay and T. Matsumoto and U. Maitra",
year = "2000",
language = "English (US)",
volume = "11",
pages = "4005--4018",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "11",

}

TY - JOUR

T1 - Fission yeast Int6 is not essential for global translation initiation, but deletion of int6+ causes hypersensitivity to caffeine and affects spore formation

AU - Bandyopadhyay, A.

AU - Matsumoto, T.

AU - Maitra, U.

PY - 2000

Y1 - 2000

N2 - Mammalian INT6 protein has been considered to be a subunit of the eukaryotic translation initiation factor, eIF3. The Int6 locus is also known as a common integration site of mouse mammary tumor virus (MMTV). However, the function of Int6 in translation initiation and the mechanism of Int6-mediated tumor induction are yet to be explored. In this study, the fission yeast, Schizosaccharomyces pombe, int6+, which is 43% identical to the mammalian counterpart, was deleted. Despite the evidence that the majority of Int6 protein was associated with 40S particles in this organism, strains lacking int6+ (Δint6) were viable and showed only moderate inhibition in the rate of in vivo global protein synthesis. Polysome profile analysis showed no apparent defects in translation initiation. Δint6 exhibited a hypersensitivity to caffeine, which could be suppressed by the addition of sorbitol to the growth medium. This and other phenotypes would imply that int6+ is required for the integrity of cell membrane. In meiosis, Δint6 produced incomplete tetrads frequently. High dosage expression of a truncated mutant of int6+ conferred a hypersensitivity to caffeine, but did not cause the defect in meiosis. A possible link between the function of int6+ and the Δint6-phenotypes is discussed.

AB - Mammalian INT6 protein has been considered to be a subunit of the eukaryotic translation initiation factor, eIF3. The Int6 locus is also known as a common integration site of mouse mammary tumor virus (MMTV). However, the function of Int6 in translation initiation and the mechanism of Int6-mediated tumor induction are yet to be explored. In this study, the fission yeast, Schizosaccharomyces pombe, int6+, which is 43% identical to the mammalian counterpart, was deleted. Despite the evidence that the majority of Int6 protein was associated with 40S particles in this organism, strains lacking int6+ (Δint6) were viable and showed only moderate inhibition in the rate of in vivo global protein synthesis. Polysome profile analysis showed no apparent defects in translation initiation. Δint6 exhibited a hypersensitivity to caffeine, which could be suppressed by the addition of sorbitol to the growth medium. This and other phenotypes would imply that int6+ is required for the integrity of cell membrane. In meiosis, Δint6 produced incomplete tetrads frequently. High dosage expression of a truncated mutant of int6+ conferred a hypersensitivity to caffeine, but did not cause the defect in meiosis. A possible link between the function of int6+ and the Δint6-phenotypes is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0033747046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033747046&partnerID=8YFLogxK

M3 - Article

VL - 11

SP - 4005

EP - 4018

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 11

ER -