TY - JOUR
T1 - Fine tuning by human CD1e of lipid-specific immune responses
AU - Facciotti, Federica
AU - Cavallari, Marco
AU - Angénieux, Catherine
AU - Garcia-Alles, Luis F.
AU - Signorino-Gelo, François
AU - Angman, Lena
AU - Gilleron, Martine
AU - Prandi, Jacques
AU - Puzo, Germain
AU - Panza, Luigi
AU - Xia, Chengfeng
AU - Wang, Peng George
AU - Dellabona, Paolo
AU - Casorati, Giulia
AU - Porcelli, Steven A.
AU - De La Salle, Henri
AU - Mori, Lucia
AU - De Libero, Gennaro
PY - 2011/8/23
Y1 - 2011/8/23
N2 - CD1e is a member of the CD1 family that participates in lipid antigen presentation without interacting with the T-cell receptor. It binds lipids in lysosomes and facilitates processing of complex glycolipids, thus promoting editing of lipid antigens. We find that CD1e may positively or negatively affect lipid presentation by CD1b, CD1c, and CD1d. This effect is caused by the capacity of CD1e to facilitate rapid formation of CD1-lipid complexes, as shown for CD1d, and also to accelerate their turnover. Similar results were obtained with antigen-presenting cells from CD1e transgenic mice in which lipid complexes are assembled more efficiently and show faster turnover than in WT antigen-presenting cells. These effects maximize and temporally narrow CD1-restricted responses, as shown by reactivity to Sphingomonas paucimobilis-derived lipid antigens. CD1e is therefore an important modulator of both group 1 and group 2 CD1-restricted responses influencing the lipid antigen availability as well as the generation and persistence of CD1-lipid complexes.
AB - CD1e is a member of the CD1 family that participates in lipid antigen presentation without interacting with the T-cell receptor. It binds lipids in lysosomes and facilitates processing of complex glycolipids, thus promoting editing of lipid antigens. We find that CD1e may positively or negatively affect lipid presentation by CD1b, CD1c, and CD1d. This effect is caused by the capacity of CD1e to facilitate rapid formation of CD1-lipid complexes, as shown for CD1d, and also to accelerate their turnover. Similar results were obtained with antigen-presenting cells from CD1e transgenic mice in which lipid complexes are assembled more efficiently and show faster turnover than in WT antigen-presenting cells. These effects maximize and temporally narrow CD1-restricted responses, as shown by reactivity to Sphingomonas paucimobilis-derived lipid antigens. CD1e is therefore an important modulator of both group 1 and group 2 CD1-restricted responses influencing the lipid antigen availability as well as the generation and persistence of CD1-lipid complexes.
KW - CD1 restriction
KW - Lipid transfer proteins
KW - Natural killer T cells
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U2 - 10.1073/pnas.1108809108
DO - 10.1073/pnas.1108809108
M3 - Article
C2 - 21844346
AN - SCOPUS:80052183234
SN - 0027-8424
VL - 108
SP - 14228
EP - 14233
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 34
ER -