Fine specificities of two lectins from Cymbosema roseum seeds: A lectin specific for high-mannose oligosaccharides and a lectin specific for blood group H type II trisaccharide

Tarun K. Dam, Benildo S. Cavada, Celso S. Nagano, Bruno A.M. Rocha, Raquel G. Benevides, Kyria S. Nascimento, Luiz A.G. De Sousa, Stefan Oscarson, C. Fred Brewer

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fuc1,2Gal1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the (1,6) Man residue, the 3- and 4-hydroxyl group of the (1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewisx, Lewisy, Lewisa and Lewisb epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe.

Original languageEnglish (US)
Pages (from-to)925-933
Number of pages9
JournalGlycobiology
Volume21
Issue number7
DOIs
StatePublished - Jul 1 2011

Keywords

  • fine specificity
  • fucose-specific lectin
  • glycans
  • mannose-specific lectin

ASJC Scopus subject areas

  • Biochemistry

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