Fine specificities of two lectins from Cymbosema roseum seeds: A lectin specific for high-mannose oligosaccharides and a lectin specific for blood group H type II trisaccharide

Tarun K. Dam, Benildo S. Cavada, Celso S. Nagano, Bruno A M Rocha, Raquel G. Benevides, Kyria S. Nascimento, Luiz A G De Sousa, Stefan Oscarson, Curtis F. Brewer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fuc1,2Gal1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the (1,6) Man residue, the 3- and 4-hydroxyl group of the (1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewisx, Lewisy, Lewisa and Lewisb epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe.

Original languageEnglish (US)
Pages (from-to)925-933
Number of pages9
JournalGlycobiology
Volume21
Issue number7
DOIs
StatePublished - Jul 2011

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Mannose-Binding Lectins
ABO Blood-Group System
Trisaccharides
Mannose
Oligosaccharides
Lectins
Seed
Seeds
Hydroxyl Radical
Polysaccharides
Epitopes
Fucose

Keywords

  • fine specificity
  • fucose-specific lectin
  • glycans
  • mannose-specific lectin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Fine specificities of two lectins from Cymbosema roseum seeds : A lectin specific for high-mannose oligosaccharides and a lectin specific for blood group H type II trisaccharide. / Dam, Tarun K.; Cavada, Benildo S.; Nagano, Celso S.; Rocha, Bruno A M; Benevides, Raquel G.; Nascimento, Kyria S.; De Sousa, Luiz A G; Oscarson, Stefan; Brewer, Curtis F.

In: Glycobiology, Vol. 21, No. 7, 07.2011, p. 925-933.

Research output: Contribution to journalArticle

Dam, Tarun K. ; Cavada, Benildo S. ; Nagano, Celso S. ; Rocha, Bruno A M ; Benevides, Raquel G. ; Nascimento, Kyria S. ; De Sousa, Luiz A G ; Oscarson, Stefan ; Brewer, Curtis F. / Fine specificities of two lectins from Cymbosema roseum seeds : A lectin specific for high-mannose oligosaccharides and a lectin specific for blood group H type II trisaccharide. In: Glycobiology. 2011 ; Vol. 21, No. 7. pp. 925-933.
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abstract = "The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the {"}core{"} trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fuc1,2Gal1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the (1,6) Man residue, the 3- and 4-hydroxyl group of the (1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewisx, Lewisy, Lewisa and Lewisb epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe.",
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AU - Cavada, Benildo S.

AU - Nagano, Celso S.

AU - Rocha, Bruno A M

AU - Benevides, Raquel G.

AU - Nascimento, Kyria S.

AU - De Sousa, Luiz A G

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AU - Brewer, Curtis F.

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AB - The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fuc1,2Gal1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the (1,6) Man residue, the 3- and 4-hydroxyl group of the (1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewisx, Lewisy, Lewisa and Lewisb epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe.

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