TY - JOUR
T1 - Fine specificities of two lectins from Cymbosema roseum seeds
T2 - A lectin specific for high-mannose oligosaccharides and a lectin specific for blood group H type II trisaccharide
AU - Dam, Tarun K.
AU - Cavada, Benildo S.
AU - Nagano, Celso S.
AU - Rocha, Bruno A.M.
AU - Benevides, Raquel G.
AU - Nascimento, Kyria S.
AU - De Sousa, Luiz A.G.
AU - Oscarson, Stefan
AU - Brewer, C. Fred
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/7
Y1 - 2011/7
N2 - The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fuc1,2Gal1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the (1,6) Man residue, the 3- and 4-hydroxyl group of the (1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewisx, Lewisy, Lewisa and Lewisb epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe.
AB - The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fuc1,2Gal1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the (1,6) Man residue, the 3- and 4-hydroxyl group of the (1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewisx, Lewisy, Lewisa and Lewisb epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe.
KW - fine specificity
KW - fucose-specific lectin
KW - glycans
KW - mannose-specific lectin
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U2 - 10.1093/glycob/cwr025
DO - 10.1093/glycob/cwr025
M3 - Article
C2 - 21406562
AN - SCOPUS:79958851866
SN - 0959-6658
VL - 21
SP - 925
EP - 933
JO - Glycobiology
JF - Glycobiology
IS - 7
ER -