Filterability of freshly-collected sickle erythrocytes under venous oxygen pressure without exposure to air

Siddharth Shah, Rhonda G. Acholonu, Kwaku Ohene-Frempong, Toshio Asakura

Research output: Contribution to journalArticle

Abstract

We previously found that blood samples collected from steady-state patients with sickle cell disease (SCD) without exposure to air contain a new type of reversibly sickled cells (RSCs) with blunt edges at a level of as high as 78%. Since partial oxygenation of once-deoxygenated sickled cells with pointy edges to near venous oxygen pressure generates similar sickled cells with blunt edges in vitro, we named them as partially oxygenated sickled cells (POSCs). On the other hand, partial deoxygenation of once-oxygenated SS cells to venous oxygen pressure generates partially deoxygenated sickled cells (PDSCs) with pointy edges. In this study, we obtained blood samples from 6 steady-state patients with SCD under venous oxygen pressure without exposure to air, subjected them to various oxygenation/deoxygenation/reoxygenation cycles, and studied their filterability through a membrane filter with pore diameter of 3. μm, the theoretical minimum diameter of a capillary. Our results indicated that discocytes, POSCs with blunt edges, and irreversibly sickled cells could deform and pass through the filter, while PDSCs with pointy edges were rigid and could not. The filterability of SS cells seems to be related to the length and amount of deoxy-hemoglobin S fibers in the cells.

Original languageEnglish (US)
Pages (from-to)328-335
Number of pages8
JournalBlood Cells, Molecules, and Diseases
Volume55
Issue number4
DOIs
StatePublished - Dec 1 2015
Externally publishedYes

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Venous Pressure
Erythrocytes
Air
Oxygen
Sickle Cell Anemia
Sickle Hemoglobin

Keywords

  • Filterability
  • Partially deoxygenated sickled cells
  • Partially oxygenated sickled cells
  • Sickle cell disease

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Hematology
  • Cell Biology
  • Medicine(all)

Cite this

Filterability of freshly-collected sickle erythrocytes under venous oxygen pressure without exposure to air. / Shah, Siddharth; Acholonu, Rhonda G.; Ohene-Frempong, Kwaku; Asakura, Toshio.

In: Blood Cells, Molecules, and Diseases, Vol. 55, No. 4, 01.12.2015, p. 328-335.

Research output: Contribution to journalArticle

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