TY - JOUR
T1 - Filterability of freshly-collected sickle erythrocytes under venous oxygen pressure without exposure to air
AU - Shah, Siddharth
AU - Acholonu, Rhonda Graves
AU - Ohene-Frempong, Kwaku
AU - Asakura, Toshio
N1 - Funding Information:
This work was supported by grants from the National Institutes of Health (grants 2P60 H13832 and U24-HL-58390 ) (T.A.) and the Mizuno Fund of The Children's Hospital of Philadelphia (T.A.).
Publisher Copyright:
© 2015.
PY - 2015/12
Y1 - 2015/12
N2 - We previously found that blood samples collected from steady-state patients with sickle cell disease (SCD) without exposure to air contain a new type of reversibly sickled cells (RSCs) with blunt edges at a level of as high as 78%. Since partial oxygenation of once-deoxygenated sickled cells with pointy edges to near venous oxygen pressure generates similar sickled cells with blunt edges in vitro, we named them as partially oxygenated sickled cells (POSCs). On the other hand, partial deoxygenation of once-oxygenated SS cells to venous oxygen pressure generates partially deoxygenated sickled cells (PDSCs) with pointy edges. In this study, we obtained blood samples from 6 steady-state patients with SCD under venous oxygen pressure without exposure to air, subjected them to various oxygenation/deoxygenation/reoxygenation cycles, and studied their filterability through a membrane filter with pore diameter of 3. μm, the theoretical minimum diameter of a capillary. Our results indicated that discocytes, POSCs with blunt edges, and irreversibly sickled cells could deform and pass through the filter, while PDSCs with pointy edges were rigid and could not. The filterability of SS cells seems to be related to the length and amount of deoxy-hemoglobin S fibers in the cells.
AB - We previously found that blood samples collected from steady-state patients with sickle cell disease (SCD) without exposure to air contain a new type of reversibly sickled cells (RSCs) with blunt edges at a level of as high as 78%. Since partial oxygenation of once-deoxygenated sickled cells with pointy edges to near venous oxygen pressure generates similar sickled cells with blunt edges in vitro, we named them as partially oxygenated sickled cells (POSCs). On the other hand, partial deoxygenation of once-oxygenated SS cells to venous oxygen pressure generates partially deoxygenated sickled cells (PDSCs) with pointy edges. In this study, we obtained blood samples from 6 steady-state patients with SCD under venous oxygen pressure without exposure to air, subjected them to various oxygenation/deoxygenation/reoxygenation cycles, and studied their filterability through a membrane filter with pore diameter of 3. μm, the theoretical minimum diameter of a capillary. Our results indicated that discocytes, POSCs with blunt edges, and irreversibly sickled cells could deform and pass through the filter, while PDSCs with pointy edges were rigid and could not. The filterability of SS cells seems to be related to the length and amount of deoxy-hemoglobin S fibers in the cells.
KW - Filterability
KW - Partially deoxygenated sickled cells
KW - Partially oxygenated sickled cells
KW - Sickle cell disease
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U2 - 10.1016/j.bcmd.2015.06.012
DO - 10.1016/j.bcmd.2015.06.012
M3 - Article
C2 - 26460256
AN - SCOPUS:84943533666
VL - 55
SP - 328
EP - 335
JO - Blood Cells, Molecules, and Diseases
JF - Blood Cells, Molecules, and Diseases
SN - 1079-9796
IS - 4
ER -