Fibronectin synthesis and degradation in human fibroblasts with aging

Jerry Shevitz, C. S.P. Jenkins, Victor B. Hatcher

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Fibronectin was measured in early and late passaged human skin fibroblasts utilizing immunoprecipitation and polyacrylamide gel electrophoresis. A progressive increase in the rate of fibronectin and total cellular protein synthesis per cell was observed by late passaged human diploid fibroblasts. The absolute protein concentration increased in the late passaged fibroblasts. There was no significant difference in the [3H]leucine incorporation into fibronectin or total cellular protein/mg protein. The turnover of fibronectin and total cellular protein did not differ between early and late passaged fibroblasts. The transport of fibronectin to the cell membrane was similar in late passaged fibroblasts. The increased fibronectin synthesis in senescent fibroblasts appeared to correlate with the general increase rate of protein synthesis/cell.

Original languageEnglish (US)
Pages (from-to)221-232
Number of pages12
JournalMechanisms of Ageing and Development
Volume35
Issue number3
DOIs
StatePublished - Aug 1986

Keywords

  • Aging
  • Fibronectin synthesis
  • Protein synthesis

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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