TY - JOUR
T1 - Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
AU - Pennacchietti, Francesca
AU - Serebrovskaya, Ekaterina O.
AU - Faro, Aline R.
AU - Shemyakina, Irina I.
AU - Bozhanova, Nina G.
AU - Kotlobay, Alexey A.
AU - Gurskaya, Nadya G.
AU - Bodén, Andreas
AU - Dreier, Jes
AU - Chudakov, Dmitry M.
AU - Lukyanov, Konstantin A.
AU - Verkhusha, Vladislav V.
AU - Mishin, Alexander S.
AU - Testa, Ilaria
N1 - Publisher Copyright:
© 2018, The Author(s).
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.
AB - Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.
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U2 - 10.1038/s41592-018-0052-9
DO - 10.1038/s41592-018-0052-9
M3 - Article
C2 - 29988095
AN - SCOPUS:85049608980
SN - 1548-7091
VL - 15
SP - 601
EP - 604
JO - Nature Methods
JF - Nature Methods
IS - 8
ER -