Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Francesca Pennacchietti, Ekaterina O. Serebrovskaya, Aline R. Faro, Irina I. Shemyakina, Nina G. Bozhanova, Alexey A. Kotlobay, Nadya G. Gurskaya, Andreas Bodén, Jes Dreier, Dmitry M. Chudakov, Konstantin A. Lukyanov, Vladislav V. Verkhusha, Alexander S. Mishin, Ilaria Testa

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.

Original languageEnglish (US)
Pages (from-to)601-604
Number of pages4
JournalNature Methods
Volume15
Issue number8
DOIs
StatePublished - Aug 1 2018

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Pennacchietti, F., Serebrovskaya, E. O., Faro, A. R., Shemyakina, I. I., Bozhanova, N. G., Kotlobay, A. A., Gurskaya, N. G., Bodén, A., Dreier, J., Chudakov, D. M., Lukyanov, K. A., Verkhusha, V. V., Mishin, A. S., & Testa, I. (2018). Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy. Nature Methods, 15(8), 601-604. https://doi.org/10.1038/s41592-018-0052-9