Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Francesca Pennacchietti, Ekaterina O. Serebrovskaya, Aline R. Faro, Irina I. Shemyakina, Nina G. Bozhanova, Alexey A. Kotlobay, Nadya G. Gurskaya, Andreas Bodén, Jes Dreier, Dmitry M. Chudakov, Konstantin A. Lukyanov, Vladislav Verkhusha, Alexander S. Mishin, Ilaria Testa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalNature Methods
DOIs
StateAccepted/In press - Jul 9 2018

Fingerprint

Viola
Methyl Green
Light
Proteins
Dosimetry
Cells
Scattering
Imaging techniques
red fluorescent protein

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Pennacchietti, F., Serebrovskaya, E. O., Faro, A. R., Shemyakina, I. I., Bozhanova, N. G., Kotlobay, A. A., ... Testa, I. (Accepted/In press). Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy. Nature Methods, 1-4. https://doi.org/10.1038/s41592-018-0052-9

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy. / Pennacchietti, Francesca; Serebrovskaya, Ekaterina O.; Faro, Aline R.; Shemyakina, Irina I.; Bozhanova, Nina G.; Kotlobay, Alexey A.; Gurskaya, Nadya G.; Bodén, Andreas; Dreier, Jes; Chudakov, Dmitry M.; Lukyanov, Konstantin A.; Verkhusha, Vladislav; Mishin, Alexander S.; Testa, Ilaria.

In: Nature Methods, 09.07.2018, p. 1-4.

Research output: Contribution to journalArticle

Pennacchietti, F, Serebrovskaya, EO, Faro, AR, Shemyakina, II, Bozhanova, NG, Kotlobay, AA, Gurskaya, NG, Bodén, A, Dreier, J, Chudakov, DM, Lukyanov, KA, Verkhusha, V, Mishin, AS & Testa, I 2018, 'Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy', Nature Methods, pp. 1-4. https://doi.org/10.1038/s41592-018-0052-9
Pennacchietti F, Serebrovskaya EO, Faro AR, Shemyakina II, Bozhanova NG, Kotlobay AA et al. Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy. Nature Methods. 2018 Jul 9;1-4. https://doi.org/10.1038/s41592-018-0052-9
Pennacchietti, Francesca ; Serebrovskaya, Ekaterina O. ; Faro, Aline R. ; Shemyakina, Irina I. ; Bozhanova, Nina G. ; Kotlobay, Alexey A. ; Gurskaya, Nadya G. ; Bodén, Andreas ; Dreier, Jes ; Chudakov, Dmitry M. ; Lukyanov, Konstantin A. ; Verkhusha, Vladislav ; Mishin, Alexander S. ; Testa, Ilaria. / Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy. In: Nature Methods. 2018 ; pp. 1-4.
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